• Featured Product
  • KD/KO Validated

MYCN Polyklonaler Antikörper

MYCN Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10159-2-AP

Synonyme

bHLHe37, MODED, MYCN, N myc, N myc proto oncogene protein, ODED



Geprüfte Anwendungen

Erfolgreiche Detektion in WBhumanes Plazenta-Gewebe, C6-Zellen, Maushirngewebe, Rattenhirngewebe, SH-SY5Y-Zellen, SKOV-3-Zellen
Erfolgreiche Detektion in IHChumanes Schilddrüsenkarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:3000
Immunhistochemie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

10159-2-AP bindet in WB, IHC, IF, ELISA MYCN und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen MYCN fusion protein Ag0193
Vollständiger Name v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian)
Berechnetes Molekulargewicht 50 kDa
Beobachtetes Molekulargewicht 65-70 kDa
GenBank-ZugangsnummerBC002712
Gene symbol MYCN
Gene ID (NCBI) 4613
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYCN, also named as BHLHE37 and NMYC, is a transcription factor. It is primarily expressed in normal developing embryos and is thought to be critical in brain and other neural development. It is often amplified in human neuroblastomas. IR34A can suppress MYCN expression, it suggest MYCN has a role in cell growth. The expression of the MCYN is upregulated in a variety of human tumors, most frequently neuroblastoma, where the level of expression appears to increase as the tumor progresses. The calculated molecuar weight of MYCN is 50 kDa, but Phosphorylated MYCN is about 65-70 kDa. MYCN exsits two isoforms and MV of isoform is respectively 65 kDa and 45 kDa. (PMID: 19615087 )

Protokolle

Produktspezifische Protokolle
WB protocol for MYCN antibody 10159-2-APProtokoll herunterladen
IHC protocol for MYCN antibody 10159-2-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseIHC

Nature

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Authors - Joshua E Burda
humanWB

Elife

Combined ALK and MDM2 inhibition increases antitumor activity and overcomes resistance in human ALK mutant neuroblastoma cell lines and xenograft models.

Authors - Hui Qin Wang
  • KD Validated
humanWB,IHC

Cancers (Basel)

Transcriptomic Analyses of MYCN-Regulated Genes in Anaplastic Wilms' Tumour Cell Lines Reveals Oncogenic Pathways and Potential Therapeutic Vulnerabilities.

Authors - Marianna Szemes
  • KD Validated
mouseIHC

Front Oncol

Downregulation of MYCN through PI3K Inhibition in Mouse Models of Pediatric Neural Cancer.

Authors - Tene Aneka Cage
humanIHC

Cancer

Neuroblastoma of undifferentiated subtype, prognostic significance of prominent nucleolar formation, and MYC/MYCN protein expression: a report from the Children's Oncology Group.

Authors - Larry L Wang
mouseWB,IHC

Front Cell Dev Biol

An E2F5-TFDP1-BRG1 Complex Mediates Transcriptional Activation of MYCN in Hepatocytes.

Authors - Zhiwen Fan

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

CHAO (Verified Customer) (03-31-2022)

Strong bands.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse Heart
FH

Tom (Verified Customer) (08-22-2020)

I saw a specific ~60 kDa band when performing western blots using this MYCN antibody. The band is bigger than molecular weight of MYCN, probably due to post-translational modifications.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse heart
FH

Tom (Verified Customer) (08-22-2020)

I performed immunofluorescence using this antibody. I observed very strong MYCN signal in mouse heart tissue sections. However, I saw cytoplasmic staining in addition to nuclear staining. It is probably due to MYCN's localization to both cytoplasm and nuclei or this antibody has some background staining.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Mouse heart
FH

Jessica (Verified Customer) (10-31-2018)

Strongest band observed was around 60 kDa with some much weaker bands much higher and much lower observable with higher exposures. Ab had affinity for BE2C cell lysate, but did not bind the c-myc expressed in Sy5Y cells on the same blot. I used 2 % skim milk PBST for incubation. As MYCN Abs go this one is pretty good!

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Neuroblastoma cell lines