MYCN Polyklonaler Antikörper

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MYCN Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10159-2-PBS

Synonyme

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  WB analysis of human placenta using 10159-2-AP (same clone as 10159-2-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10159-2-AP (MYCN antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10159-2-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 10159-2-AP (same clone as 10159-2-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10159-2-AP (MYCN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10159-2-PBS in a different storage buffer formulation.

• 

  WB analysis of SKOV-3 using 10159-2-AP (same clone as 10159-2-PBS)

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10159-2-AP (MYCN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10159-2-PBS in a different storage buffer formulation.

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  WB analysis of C6 using 10159-2-AP (same clone as 10159-2-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 10159-2-AP (MYCN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10159-2-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 10159-2-AP (same clone as 10159-2-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10159-2-AP (MYCN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10159-2-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 10159-2-AP (same clone as 10159-2-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10159-2-AP (MYCN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10159-2-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 10159-2-AP (same clone as 10159-2-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10159-2-AP (MYCN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10159-2-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 10159-2-AP (same clone as 10159-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10159-2-AP (MYCN Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10159-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 10159-2-AP (same clone as 10159-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10159-2-AP (MYCN Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10159-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10159-2-PBS bindet in WB, IHC, Indirect ELISA MYCN und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MYCN fusion protein Ag0193
• Vollständiger Name: v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian)
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC002712
• Gene symbol: MYCN
• Gene ID (NCBI): 4613
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYCN, also named as BHLHE37 and NMYC, is a transcription factor. It is primarily expressed in normal developing embryos and is thought to be critical in brain and other neural development. It is often amplified in human neuroblastomas. IR34A can suppress MYCN expression, it suggest MYCN has a role in cell growth. The expression of the MCYN is upregulated in a variety of human tumors, most frequently neuroblastoma, where the level of expression appears to increase as the tumor progresses. The calculated molecuar weight of MYCN is 50 kDa, but Phosphorylated MYCN is about 65-70 kDa. MYCN exsits two isoforms and MV of isoform is respectively 65 kDa and 45 kDa. (PMID: 19615087 )

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