MYCN Rekombinanter Antikörper

MYCN Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

242406D8

Kat-Nr. : 84886-3-RR

Synonyme

242406D8, bHLHe37, MODED, N myc, N myc proto oncogene protein

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  WB analysis using 84886-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84886-3-RR (MYCN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 84886-3-RR

  human placenta tissue were subjected to SDS PAGE followed by western blot with 84886-3-RR (MYCN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  FC experiment of SH-SY5Y using 84886-3-RR

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug MYCN Recombinant antibody (84886-3-RR, Clone:242406D8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: IMR-32 cells, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in FC (Intra): SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84886-3-RR bindet in WB, FC (Intra), ELISA MYCN und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MYCN fusion protein Ag27224
• Vollständiger Name: v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian)
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: ~65 kDa
• GenBank-Zugangsnummer: BC002712
• Gene symbol: MYCN
• Gene ID (NCBI): 4613
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYCN, also named as BHLHE37 and NMYC, is a transcription factor. It is primarily expressed in normal developing embryos and is thought to be critical in brain and other neural development. It is often amplified in human neuroblastomas. IR34A can suppress MYCN expression, it suggest MYCN has a role in cell growth. The expression of the MCYN is upregulated in a variety of human tumors, most frequently neuroblastoma, where the level of expression appears to increase as the tumor progresses. The calculated molecuar weight of MYCN is 50 kDa, but Phosphorylated MYCN is about 65-70 kDa. MYCN exsits two isoforms and MV of isoform is respectively 65 kDa and 45 kDa. (PMID: 19615087 )