MYD88 Monoklonaler Antikörper

MYD88 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

8

CloneNo.

1B10E12

Kat-Nr. : 66660-1-Ig

Synonyme

MYD88, MYD88D

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Galerie der Validierungsdaten

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  WB analysis using 66660-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66660-1-Ig (MYD88 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  WB analysis using 66660-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66660-1-Ig (MYD88 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HSC-T6 cells, 4T1-Zellen, HepG2-Zellen, NIH/3T3-Zellen, PC-12-Zellen, Sp2/0-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IHC: See 1 publications below

Produktinformation

66660-1-Ig bindet in WB, IHC, ELISA MYD88 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MYD88 fusion protein Ag19770
• Vollständiger Name: myeloid differentiation primary response gene (88)
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 33-36 kDa
• GenBank-Zugangsnummer: BC013589
• Gene symbol: MYD88
• Gene ID (NCBI): 4615
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Background

Myeloid differentiation primary response 88 (MYD88) is an adapter protein critical to the innate and adaptive immune response.

What is the molecular weight of MYD88?

The molecular weight of MYD88 is 33 kDa.

What is the cellular localization of MYD88?

The subcellular localization of MYD88 is largely confined to the cytoplasm as condensed forms or aggregated structures.

What is the role of MYD88 in the IL-1R signaling pathway?

MYD88 plays a major role in the inflammatory signaling pathways downstream of Toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) families. MYD88 links these receptors to IL-1R-associated kinases (IRAK), such as IRAK1 and IRAK2, via protein-protein interactions. The C-terminal TIR domain of MYD88 mediates the interaction with the receptors, whereas the N-terminal death domain of MYD88 associates with IRAK family members (PMID: 25580251). MYD88 acts via its intermediate domain to phosphorylate and thus activate IRAK1, IRAK2, IRF7, and TRAF6 to trigger NF-kappa-B signaling and cytokine secretion as part of the inflammatory response (PMID: 19679662).

What is MYD88's involvement in disease?

Defects in MYD88 due to deficiency of the protein leads to recurrent pyogenic bacterial infections, including invasive pneumococcal disease. Patients usually die between 1 and 11 months of age, but surviving patients are otherwise healthy with normal resistance to other microbes (PMID: 18669862). Mutations in the MYD88 gene also lead to the development of cancers such as lymphoma (PMID: 21179087) and some autoimmune disorders like ulcerative colitis (PMID: 24189845).

Publikationen

Species	Application	Title
mouse	WB	ACS Nano; Bacteria-Anchoring Hybrid Liposome Capable of Absorbing Multiple Toxins for Antivirulence Therapy of Escherichia coli Infection.; Authors - Lixian Jiang
mouse	WB	Theranostics; Fibrinogen-like protein 2 aggravates nonalcoholic steatohepatitis via interaction with TLR4, eliciting inflammation in macrophages and inducing hepatic lipid metabolism disorder.; Authors - Junjian Hu
mouse	WB	Food Funct; Salvianolic acid B prevents body weight gain and regulates gut microbiota and LPS/TLR4 signaling pathway in high-fat diet-induced obese mice.; Authors - Lin Li
mouse	WB	Pharm Biol; Catalpol relieved angiotensin II-induced blood–brain barrier destruction via inhibiting the TLR4 pathway in brain endothelial cells; Authors - Yu Xia
mouse	WB	Life Sci; Salvianolate reduces neuronal apoptosis by suppressing OGD-induced microglial activation.; Authors - Pengwei Luan
mouse	WB	Oncol Rep; α7‑nAchR agonist GTS‑21 reduces radiation‑induced lung injury.; Authors - Zijie Mei