MYD88 Monoklonaler Antikörper

MYD88 Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(65)

CloneNo.

2E10D3

Kat-Nr. : 67969-1-Ig

Synonyme

2E10D3

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  WB analysis using 67969-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67969-1-Ig (MYD88 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  WB analysis of HL-60 using 67969-1-Ig

  HL-60 cells were subjected to SDS PAGE followed by western blot with 67969-1-Ig (MYD88 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 67969-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67969-1-Ig (MYD88 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67969-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67969-1-Ig (MYD88 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig spleen using 67969-1-Ig

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 67969-1-Ig (MYD88 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rabbit spleen using 67969-1-Ig

  rabbit spleen tissue were subjected to SDS PAGE followed by western blot with 67969-1-Ig (MYD88 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 67969-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67969-1-Ig (MYD88 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 67969-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67969-1-Ig (MYD88 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 67969-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67969-1-Ig (MYD88 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 67969-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67969-1-Ig (MYD88 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse liver using 67969-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using MYD88 antibody (67969-1-Ig, Clone: 2E10D3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of A431 using 67969-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using MYD88 antibody (67969-1-Ig, Clone: 2E10D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HL-60-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen, Hausschwein-Milzgewebe, Kaninchenmilzgewebe, Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mauslebergewebe
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:3000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 54 publications below
• IHC: See 11 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

67969-1-Ig bindet in WB, IHC, IF/ICC, IF-P, IP, ELISA MYD88 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MYD88 fusion protein Ag27659
• Vollständiger Name: myeloid differentiation primary response gene (88)
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC013589
• Gene symbol: MYD88
• Gene ID (NCBI): 4615
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYD88 is a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. MYD88 is the key adaptor protein for the interleukin-1 receptor (IL-1R) and most Toll-like receptors (TLRs), which are essential for innate immunity and pathogen-associated molecular pattern recognition. Mutations in the MyD88 gene lead to the development of cancer in humans and mice suggesting that MyD88 also plays a cell autonomous role in tissue homeostasis.

Publikationen

Species	Application	Title
rat	WB	J Control Release; Controlled extracellular vesicles release from aminoguanidine nanoparticle-loaded polylysine hydrogel for synergistic treatment of spinal cord injury; Authors - Shaoke Wang
mouse,human	WB	Phytomedicine; Rice bran active peptide (RBAP) inhibited macrophage differentiation to foam cell and atherosclerosis in mice via regulating cholesterol efflux; Authors - Jianfei Mu
mouse	WB	Respir Res; Bhlhe40 deficiency attenuates LPS-induced acute lung injury through preventing macrophage pyroptosis; Authors - Xingxing Hu
rat	WB	Inflammation; Chlorogenic Acid Alleviates Hepatic Ischemia-Reperfusion Injury by Inhibiting Oxidative Stress, Inflammation, and Mitochondria-Mediated Apoptosis In Vivo and In Vitro; Authors - Kai Li
mouse	WB	Front Bioeng Biotechnol; Polyvinylpyrrolidone-Modified Taxifolin Liposomes Promote Liver Repair by Modulating Autophagy to Inhibit Activation of the TLR4/NF-κB Signaling Pathway.; Authors - Qiteng Ding
mouse	WB	Front Pharmacol; Ephedra sinica polysaccharide regulate the anti-inflammatory immunity of intestinal microecology and bacterial metabolites in rheumatoid arthritis; Authors - Yanmiao Ma