MYD88 Monoklonaler Antikörper

MYD88 Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

2E10D3

Kat-Nr. : 67969-1-PBS

Synonyme

2E10D3

---

• 

  WB analysis using 67969-1-Ig (same clone as 67969-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67969-1-Ig (MYD88 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67969-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HL-60 using 67969-1-Ig (same clone as 67969-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 67969-1-Ig (MYD88 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67969-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HSC-T6 using 67969-1-Ig (same clone as 67969-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67969-1-Ig (MYD88 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67969-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 67969-1-Ig (same clone as 67969-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67969-1-Ig (MYD88 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67969-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig spleen using 67969-1-Ig (same clone as 67969-1-PBS)

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 67969-1-Ig (MYD88 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67969-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rabbit spleen using 67969-1-Ig (same clone as 67969-1-PBS)

  rabbit spleen tissue were subjected to SDS PAGE followed by western blot with 67969-1-Ig (MYD88 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67969-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 67969-1-Ig (same clone as 67969-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67969-1-Ig (MYD88 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67969-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 67969-1-Ig (same clone as 67969-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67969-1-Ig (MYD88 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67969-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 67969-1-Ig (same clone as 67969-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67969-1-Ig (MYD88 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67969-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 67969-1-Ig (same clone as 67969-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67969-1-Ig (MYD88 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67969-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse liver using 67969-1-Ig (same clone as 67969-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using MYD88 antibody (67969-1-Ig, Clone: 2E10D3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67969-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 67969-1-Ig (same clone as 67969-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using MYD88 antibody (67969-1-Ig, Clone: 2E10D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67969-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

67969-1-PBS bindet in WB, IHC, IF/ICC, IF-P, ELISA MYD88 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MYD88 fusion protein Ag27659
• Vollständiger Name: myeloid differentiation primary response gene (88)
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC013589
• Gene symbol: MYD88
• Gene ID (NCBI): 4615
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYD88 is a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. MYD88 is the key adaptor protein for the interleukin-1 receptor (IL-1R) and most Toll-like receptors (TLRs), which are essential for innate immunity and pathogen-associated molecular pattern recognition. Mutations in the MyD88 gene lead to the development of cancer in humans and mice suggesting that MyD88 also plays a cell autonomous role in tissue homeostasis.