MYDGF/C19orf10 Monoklonaler Antikörper

MYDGF/C19orf10 Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1H8F4

Kat-Nr. : 68131-1-PBS

Synonyme

C19orf10, MYDGF, Myeloid-derived growth factor, SF20, UPF0556 protein C19orf10

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  WB analysis using 68131-1-Ig (same clone as 68131-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68131-1-Ig (MYDGF/C19orf10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68131-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 68131-1-Ig (same clone as 68131-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68131-1-Ig (MYDGF/C19orf10 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68131-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 68131-1-Ig (same clone as 68131-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68131-1-Ig (MYDGF/C19orf10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68131-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68131-1-Ig (same clone as 68131-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MYDGF/C19orf10 antibody (68131-1-Ig, Clone: 1H8F4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68131-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 68131-1-Ig (same clone as 68131-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human MYDGF/C19orf10 (68131-1-Ig, Clone:1H8F4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68131-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68131-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA MYDGF/C19orf10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MYDGF/C19orf10 fusion protein Ag13438
• Vollständiger Name: chromosome 19 open reading frame 10
• Berechnetes Molekulargewicht: 173 aa, 19 kDa
• Beobachtetes Molekulargewicht: 15-19 kDa
• GenBank-Zugangsnummer: BC010129
• Gene symbol: MYDGF/C19orf10
• Gene ID (NCBI): 56005
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Myeloid-derived growth factor (MYDGF), also named as chromosome 19 open reading frame 10 (C19orf10), is an FLS-derived protein that is secreted into the synovial fluid. MYDGF as a paracrine-acting protein secreted from monocytes/macrophages that improves tissue repair and heart function after myocardial infarction. MYDGF has been reported to regulates neutrophil motility in interstitial tissue damage(PMID: 34047769).