MYEOV Polyklonaler Antikörper

MYEOV Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 11151-1-AP

Synonyme

MYEOV, OCIM, Oncogene in multiple myeloma

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  WB analysis of SGC-7901 using 11151-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 11151-1-AP (MYEOV antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11151-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11151-1-AP (MYEOV Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 11151-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11151-1-AP (MYEOV antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SGC-7901-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 2 publications below

Produktinformation

11151-1-AP bindet in WB, IHC, ELISA MYEOV und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MYEOV fusion protein Ag1498
• Vollständiger Name: myeloma overexpressed (in a subset of t(11;14) positive multiple myelomas)
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 55-60 kDa
• GenBank-Zugangsnummer: BC011815
• Gene symbol: MYEOV
• Gene ID (NCBI): 26579
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYEOV, also named as OCIM, was originally isolated by the application of the NIH/3T3 tumorigenicity assay with DNA from a gastric carcinoma. It is a prognostic factor for patients with multiple myeloma, in part through a role of MYEOV in the control of MMC proliferation. MYEOV is a candidate oncogene activated in the amplification core located proximal toCCND1. The observed MW of this protein is 55 kDa(PMID: 20854874 ).

Publikationen

Species	Application	Title
human	IHC	Cell Oncol (Dordr); Genomic analysis and filtration of novel prognostic biomarkers based on metabolic and immune subtypes in pancreatic cancer; Authors - Guangyu Chen
human	IHC	Cell Cycle; Overexpression of MYEOV predicting poor prognosis in patients with pancreatic ductal adenocarcinoma.; Authors - Rui Tang
human	WB	Exp Hematol; MYEOV is a prognostic factor in multiple myeloma.; Authors - Moreaux Jerome J
human	WB	Cancers (Basel); Identification of MYEOV-Associated Gene Network as a Potential Therapeutic Target in Pancreatic Cancer; Authors - Yu Chen