MYH1 Monoklonaler Antikörper

MYH1 Monoklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1G10H9

Kat-Nr. : 67299-1-PBS

Synonyme

1G10H9, MyHC-2x, MyHC-IIx/d, Myosin 1, Myosin-1

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  WB analysis of human skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

  1 μg human skeletal muscle lysates were subjected to SDS PAGE followed by western blot with 67299-1-Ig (MYH1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67299-1-PBS in a different storage buffer formulation.

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  WB analysis of pig skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

  1 μg pig skeletal muscle lysates were subjected to SDS PAGE followed by western blot with 67299-1-Ig (MYH1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67299-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

  1 μg mouse skeletal muscle lysates were subjected to SDS PAGE followed by western blot with 67299-1-Ig (MYH1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67299-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67299-1-Ig (MYH1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67299-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67299-1-Ig (MYH1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67299-1-PBS in a different storage buffer formulation.

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  IHC staining of rat skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 67299-1-Ig (MYH1 antibody) at dilution of 1:80000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67299-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using MYH1 antibody (67299-1-Ig, Clone: 1G10H9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67299-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67299-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA MYH1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MYH1 fusion protein Ag17129
• Vollständiger Name: myosin, heavy chain 1, skeletal muscle, adult
• Berechnetes Molekulargewicht: 1939 aa, 223 kDa
• Beobachtetes Molekulargewicht: 220 kDa
• GenBank-Zugangsnummer: BC114545
• Gene symbol: MYH1
• Gene ID (NCBI): 4619
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYH1 (MyHC-2x) encodes the IIX isoform of myosin heavy chain (MyHC). Myosin is a large, ubiquitous, motor protein that generates force through its interaction with actin, thus involving it in a number of cellular processes including cytokinesis, karyokinesis, cell migration, and muscle contraction. Muscle fibers can be divided as type 1 (slow) and type 2 (fast). MYH1 belongs to type 2.