MYH10 Monoklonaler Antikörper

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MYH10 Monoklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2H8G7

Kat-Nr. : 67243-1-PBS

Synonyme

MYH10, Myosin 10, Myosin heavy chain 10

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  WB analysis of mouse brain using 67243-1-Ig (same clone as 67243-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67243-1-Ig (MYH10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67243-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67243-1-Ig (same clone as 67243-1-PBS)

  WB result of MYH10 antibody (67243-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYH10 transfected HEK-293 cells. This data was developed using the same antibody clone with 67243-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse cerebellum using 67243-1-Ig (same clone as 67243-1-PBS)

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 67243-1-Ig (MYH10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67243-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 67243-1-Ig (same clone as 67243-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67243-1-Ig (MYH10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67243-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat cerebellum using 67243-1-Ig (same clone as 67243-1-PBS)

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 67243-1-Ig (MYH10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67243-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 67243-1-Ig (same clone as 67243-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67243-1-Ig (MYH10 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67243-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 67243-1-Ig (same clone as 67243-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67243-1-Ig (MYH10 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67243-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse cerebellum using 67243-1-Ig (same clone as 67243-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using MYH10 antibody (67243-1-Ig, Clone: 2H8G7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67243-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67243-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA MYH10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MYH10 fusion protein Ag17069
• Vollständiger Name: myosin, heavy chain 10, non-muscle
• Berechnetes Molekulargewicht: 1985 aa, 230 kDa
• Beobachtetes Molekulargewicht: 200-230 kDa
• GenBank-Zugangsnummer: BC150634
• Gene symbol: MYH10
• Gene ID (NCBI): 4628
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYH10 (non-muscle II-b, NM IIB) is a member of non-muscle myosin II which plays fundamental roles in the maintenance of cell morphology, cell adhesion, and migration, as well as cell division. MYH10 is mainly present in nerve cells, megakaryocytes, and other non-muscle cells. It has been reported to mediate centrosome reorientation during cell migration and contribute to ciliogenesis. Overexpression of MYH10 has been observed in breast cancer.