MYH10 Rekombinanter Antikörper

MYH10 Rekombinant Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

250791D5

Kat-Nr. : 86157-3-RR

Synonyme

Myosin 10, Myosin heavy chain 10, Myosin heavy chain, non-muscle IIb, Myosin-10, NMMHC II-b

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  WB analysis using 86157-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86157-3-RR (MYH10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 86157-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86157-3-RR (MYH10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 86157-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 86157-3-RR (MYH10 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 86157-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 86157-3-RR (MYH10 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse cerebellum using 86157-3-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using MYH10 antibody (86157-3-RR, Clone: 250791D5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 86157-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86157-3-RR against Human MYH10 were performed. The affinity constant is 4.58 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293T-Zellen, Maushirngewebe, Maus-Cerebellum-Gewebe, NIH/3T3-Zellen, Rattenhirngewebe, Ratten-Cerebellum-Gewebe
• Erfolgreiche Detektion in IHC: Maus-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Cerebellum-Gewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)-P: IF-P : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86157-3-RR bindet in WB, IHC, IF-P, ELISA MYH10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MYH10 fusion protein Ag16113
• Vollständiger Name: myosin, heavy chain 10, non-muscle
• Berechnetes Molekulargewicht: 1985 aa, 230 kDa
• Beobachtetes Molekulargewicht: 229 kDa
• GenBank-Zugangsnummer: BC150634
• Gene symbol: MYH10
• Gene ID (NCBI): 4628
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYH10 (non-muscle II-b, NM IIB) is a member of non-muscle myosin II which plays fundamental roles in the maintenance of cell morphology, cell adhesion, and migration, as well as cell division. MYH10 is mainly present in nerve cells, megakaryocytes, and other non-muscle cells. It has been reported to mediate centrosome reorientation during cell migration and contribute to ciliogenesis. Overexpression of MYH10 has been observed in breast cancer.