MYH10-Specific Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MYH10-Specific Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(18)

Rezensionen (1)

Kat-Nr. : 19673-1-AP

Synonyme

Myosin 10, MYH10, NMMHC II-b, Myosin-10, Myosin heavy chain, non-muscle IIb

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  WB analysis using 19673-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19673-1-AP (MYH10-Specific antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 19673-1-AP

  WB result of MYH10-Specific antibody (19673-1-AP; 1:80000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYH10-Specific transfected HEK-293 cells.

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  IP experiment of mouse brain using 19673-1-AP

  IP result of anti-MYH10-Specific (IP:19673-1-AP, 4ug; Detection:19673-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human cerebellum using 19673-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 19673-1-AP (MYH10-Specific Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human cerebellum using 19673-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 19673-1-AP (MYH10-Specific Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human brain using 19673-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 19673-1-AP (MYH10-Specific Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 19673-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 19673-1-AP (MYH10-Specific Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 19673-1-AP

  Immunofluorescent analysis of HepG2 cells, using MYH10 antibody 19673-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HepG2 using 19673-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MYH10-Specific antibody (19673-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HEK-293-Zellen, HeLa-Zellen, Maushirngewebe, NIH/3T3-Zellen, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Cerebellum-Gewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:20000-1:100000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 16 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• IP: See 2 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

19673-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA MYH10-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: myosin, heavy chain 10, non-muscle
• Berechnetes Molekulargewicht: 231 kDa
• Beobachtetes Molekulargewicht: 229 kDa
• GenBank-Zugangsnummer: NM_005964
• Gene symbol: MYH10
• Gene ID (NCBI): 4628
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYH10 is cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. The antibody is specific to the C-term of MYH10.

Publikationen

Species	Application	Title
human	WB	Sci Adv; p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.; Authors - Xiaochao Tan
rat	WB,CoIP	Bioact Mater; Nidogen1-enriched extracellular vesicles accelerate angiogenesis and bone regeneration by targeting Myosin-10 to regulate endothelial cell adhesion.; Authors - Pengzhen Cheng
human	WB	J Am Heart Assoc; Magnesium Sulfate-Mediated Vascular Relaxation and Calcium Channel Activity in Placental Vessels Different From Nonplacental Vessels.; Authors - Jiaqi Tang
mouse	WB,IHC	Sci Rep; Disulfidptosis links the pathophysiology of ulcerative colitis and immune infiltration in colon adenocarcinoma; Authors - Chenhao Jiang
human	IHC	Clin Genet; Mutations of MYH14 Are Associated to Anorectal Malformations with Recto-perineal Fistulas in a Small Subset of Chinese Population.; Authors - Zhongxian Zhu
mouse	WB	Sci Rep; Angiotensin II-mediated MYH9 downregulation causes structural and functional podocyte injury in diabetic kidney disease.; Authors - Jeong Suk Kang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ryan (Verified Customer) (02-27-2019)

Fish had been fixed in PFA then preserved in methanol, goat serum blocking solution, 1 in 200 dilution of Myh10 and 1 in 200 dilution of Cy3 secondary and no other treatments were necessary

• Applications: Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Zebrafish Heart

MYH10-Specific Antibody Immunohistochemistry,Immunofluorescence, validation (1:200 dilution) in Zebrafish Heart (Cat no:19673-1-AP)