SMMHC Polyklonaler Antikörper

SMMHC Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Kat-Nr. : 18569-1-AP

Synonyme

MYH11, Myosin-11, Myosin heavy chain 11, Myosin 11, KIAA0866

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  WB analysis of mouse colon using 18569-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 18569-1-AP (SMMHC antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse uterus using 18569-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 18569-1-AP (SMMHC antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse bladder using 18569-1-AP

  mouse bladder tissue were subjected to SDS PAGE followed by western blot with 18569-1-AP (SMMHC antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat uterus using 18569-1-AP

  rat uterus tissue were subjected to SDS PAGE followed by western blot with 18569-1-AP (SMMHC antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human normal colon using 18569-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 18569-1-AP (SMMHC antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Kolongewebe, Maus-Blasengewebe, Maus-Uterusgewebe, Ratten-Uterusgewebe
• Erfolgreiche Detektion in IHC: human normal colon; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

18569-1-AP bindet in WB, IHC, IF, ELISA SMMHC und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SMMHC fusion protein Ag13391
• Vollständiger Name: myosin, heavy chain 11, smooth muscle
• Berechnetes Molekulargewicht: 1938 aa, 224 kDa
• Beobachtetes Molekulargewicht: 227 kDa
• GenBank-Zugangsnummer: BC101677
• Gene symbol: SMMHC/MYH11
• Gene ID (NCBI): 4629
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	J Am Heart Assoc; LINC01278 Sponges miR-500b-5p to Regulate the Expression of ACTG2 to Control Phenotypic Switching in Human Vascular Smooth Muscle Cells During Aortic Dissection.; Authors - Weitie Wang
rat	WB	Front Pharmacol; Theaflavin-3,3'-Digallate from Black Tea Inhibits Neointima Formation Through Suppression of the PDGFRβ Pathway in Vascular Smooth Muscle Cells.; Authors - Yichen Wu
human	WB	Front Med (Lausanne); Depiction of Aging-Based Molecular Phenotypes With Diverse Clinical Prognosis and Immunological Features in Gastric Cancer.; Authors - Fang He
human	IHC	BMC Cancer; Identification of a mitophagy-related gene signature for predicting overall survival and response to immunotherapy in rectal cancer; Authors - Jian Yang
human	WB	Ultrasound Med Biol; 5-Aminolevulinic Acid-Mediated Sonodynamic Therapy Promotes Phenotypic Switching from Dedifferentiated to Differentiated Phenotype via Reactive Oxygen Species and p38 Mitogen-Activated Protein Kinase in Vascular Smooth Muscle Cells.; Authors - Juhua Dan
mouse	IHC	Cell Signal; Sphingosine 1-phosphate receptor 2 promotes erythrocyte clearance by vascular smooth muscle cells in intraplaque hemorrhage through MFG-E8 production.; Authors - Daorong Pan