MYH14 Polyklonaler Antikörper

MYH14 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(16)

Kat-Nr. : 20716-1-AP

Synonyme

Myosin 14, myosin, MHC16, KIAA2034, FP17425

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  WB analysis using 20716-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20716-1-AP (MYH14 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 20716-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 20716-1-AP (MYH14 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 20716-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20716-1-AP (MYH14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 20716-1-AP

  IP result of anti-MYH14 (IP:20716-1-AP, 4ug; Detection:20716-1-AP 1:2000) with mouse kidney tissue lysate 4800ug.

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  IHC staining of human stomach cancer using 20716-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20716-1-AP (MYH14 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 20716-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using MYH14 antibody (20716-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, HEK-293-Zellen, humanes Skelettmuskelgewebe, Maus-Kolongewebe, Mausnierengewebe, Rattennierengewebe
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 12 publications below

Produktinformation

20716-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA MYH14 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Frosch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: myosin, heavy chain 14
• Berechnetes Molekulargewicht: 228 kDa
• Beobachtetes Molekulargewicht: 228 kDa
• GenBank-Zugangsnummer: NM_024729
• Gene symbol: MYH14
• Gene ID (NCBI): 79784
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYH14, also named as KIAA2034 and NMHC II-C, is cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Defects in MYH14 are the cause of deafness autosomal dominant type 4 (DFNA4). The antibody is specific to MYH14.

Publikationen

Species	Application	Title
mouse	WB	Sci Adv; Promoting the activation of T cells with glycopolymer-modified dendritic cells by enhancing cell interactions.; Authors - Liyin Yu
human	WB	J Cell Biol; Cingulin and paracingulin tether myosins-2 to junctions to mechanoregulate the plasma membrane; Authors - Florian Rouaud
human	IHC	Clin Genet; Mutations of MYH14 Are Associated to Anorectal Malformations with Recto-perineal Fistulas in a Small Subset of Chinese Population.; Authors - Zhongxian Zhu
human	IF	Stem Cells Int; Pyridoxal-5'-Phosphate Promotes Immunomodulatory Function of Adipose-Derived Mesenchymal Stem Cells through Indoleamine 2,3-Dioxygenase-1 and TLR4/NF-κB Pathway.; Authors - Cong Li
mouse	IF	Mol Biol Cell; Nonmuscle myosin-2 contractility-dependent actin turnover limits the length of epithelial microvilli.; Authors - Colbie R Chinowsky
mouse	WB,IF	Life (Basel); Mass Spectrometric Profiling of Extraocular Muscle and Proteomic Adaptations in the mdx-4cv Model of Duchenne Muscular Dystrophy.; Authors - Stephen Gargan