MYH14 Polyklonaler Antikörper

MYH14 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20716-1-PBS

Synonyme

Myosin 14, myosin, MHC16, KIAA2034, FP17425

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  WB analysis using 20716-1-AP (same clone as 20716-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20716-1-AP (MYH14 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20716-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse colon using 20716-1-AP (same clone as 20716-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 20716-1-AP (MYH14 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20716-1-PBS in a different storage buffer formulation.

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  WB analysis of human skeletal muscle using 20716-1-AP (same clone as 20716-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20716-1-AP (MYH14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20716-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse kidney using 20716-1-AP (same clone as 20716-1-PBS)

  IP result of anti-MYH14 (IP:20716-1-AP, 4ug; Detection:20716-1-AP 1:2000) with mouse kidney tissue lysate 4800ug. This data was developed using the same antibody clone with 20716-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 20716-1-AP (same clone as 20716-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20716-1-AP (MYH14 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20716-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 20716-1-AP (same clone as 20716-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using MYH14 antibody (20716-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 20716-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

20716-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA MYH14 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: myosin, heavy chain 14
• Berechnetes Molekulargewicht: 228 kDa
• Beobachtetes Molekulargewicht: 228 kDa
• GenBank-Zugangsnummer: NM_024729
• Gene symbol: MYH14
• Gene ID (NCBI): 79784
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYH14, also named as KIAA2034 and NMHC II-C, is cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Defects in MYH14 are the cause of deafness autosomal dominant type 4 (DFNA4). The antibody is specific to MYH14.