MYH14 Monoklonaler Antikörper

MYH14 Monoklonal Antikörper für FC, IF, IHC, WB,ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

1B4C9

Kat-Nr. : 66825-1-Ig

Synonyme

DFNA4, KIAA2034, MHC16, MYH14, myosin, Myosin 14, Myosin heavy chain 14, myosin, heavy chain 14, NMHC II C

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Galerie der Validierungsdaten

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  WB analysis of COLO 320 using 66825-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66825-1-Ig (MYH14 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-29 using 66825-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 66825-1-Ig (MYH14 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 66825-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66825-1-Ig (MYH14 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 66825-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66825-1-Ig (MYH14 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 66825-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66825-1-Ig (MYH14 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 66825-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66825-1-Ig (MYH14 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse skeletal muscle using 66825-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using 66825-1-Ig (MYH14 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HT-29 using 66825-1-Ig

  1X10^6 HT-29 cells were intracellularly stained with 0.2 ug Anti-Human MYH14 (66825-1-Ig, Clone:1B4C9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, HT-29-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, Maus-Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in FC: HT-29-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:100000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 2 publications below

Produktinformation

66825-1-Ig bindet in WB, IHC, IF, FC, ELISA MYH14 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MYH14 fusion protein Ag28063
• Vollständiger Name: myosin, heavy chain 14
• Berechnetes Molekulargewicht: 228 kDa
• Beobachtetes Molekulargewicht: 200-230 kDa
• GenBank-Zugangsnummer: NM_001077186
• Gene symbol: MYH14
• Gene ID (NCBI): 79784
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYH14, also named as KIAA2034 and NMHC II-C, is cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Defects in MYH14 are the cause of deafness autosomal dominant type 4 (DFNA4).

Publikationen

Species	Application	Title
rat	WB,IF	J Cell Sci; Pushing myelination: developmental regulation of myosin expression drives oligodendrocyte morphological differentiation; Authors - Helena Sofia Domingues ; KD Validated
	IF	J Cell Sci; Pushing myelination: developmental regulation of myosin expression drives oligodendrocyte morphological differentiation.; Authors - Helena Sofia Domingues