MYH2-specific Polyklonaler Antikörper

MYH2-specific Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (4)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 55069-1-AP

Synonyme

Myosin 2, MYH2, Myosin heavy chain 2, MYHSA2, MyHC-IIa

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  WB analysis of multi-cells/tissue using 55069-1-AP

  WB results of 55069-1-AP (anti-MYH2).

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  WB analysis using 55069-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55069-1-AP (MYH2-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 55069-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 55069-1-AP (MYH2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 55069-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55069-1-AP (MYH2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skeletal muscle using 55069-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55069-1-AP (MYH2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human bladder using 55069-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder using 55069-1-AP (MYH2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human bladder using 55069-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder using 55069-1-AP (MYH2 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Multizellen/-gewebe, humanes Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Skelettmuskelgewebe, humanes Blasengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

55069-1-AP bindet in WB, IHC, IF, ELISA MYH2-specific und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Hausschwein, Hund, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: myosin, heavy chain 2, skeletal muscle, adult
• Berechnetes Molekulargewicht: 223 kDa
• Beobachtetes Molekulargewicht: 200 kDa
• GenBank-Zugangsnummer: NM_017534
• Gene symbol: MYH2
• Gene ID (NCBI): 4620
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYH2 (Myosin II) is a member of the class II or conventional myosin heavy chains. Myosin II was first isolated from muscle but is also found in non muscle cells, and it is especially enriched in highly motile cell types such as amoebae. It forms bipolar filaments that interact with actin filaments to produce contraction. And it is the motor protein that generates force to drive muscle contraction. Functions in skeletal muscle contraction. The antibody is specific to MYH2, will not bind other myosins. It is a skeletal muscle specific antibody.

Publikationen

Species	Application	Title
mouse	WB	Int J Mol Sci; Loss of Myostatin Alters Mitochondrial Oxidative Phosphorylation, TCA Cycle Activity, and ATP Production in Skeletal Muscle; Authors - Xueqiao Wang
pig	WB	BMC Genomics; Identification of different myofiber types in pigs muscles and construction of regulatory networks; Authors - Chenchen Li
canine	IHC	Animals (Basel); Comparative Analysis of Muscle Fibers in Selected Muscles of Working and Companion Dog Breeds; Authors - Cezary Osiak-Wicha
mouse	WB	J Muscle Res Cell Motil; The influences of ARHGEF9 on myoblasts migration and differentiation; Authors - Shuang Li
rat	IF	Sci Rep; Dietary supplementation with inulin improves burn-induced skeletal muscle atrophy by regulating gut microbiota disorders; Authors - Shan Gao