MYH4-Specific Polyklonaler Antikörper

MYH4-Specific Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(39)

Kat-Nr. : 20140-1-AP

Synonyme

MYH2B, MYH4, MYH4-Specific, MyHC 2B, MyHC IIb, Myosin 4, Myosin heavy chain 2b, Myosin heavy chain 4, Myosin heavy chain IIb

---

• 

  WB analysis of human skeletal muscle using 20140-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20140-1-AP (MYH4-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20140-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20140-1-AP (MYH4-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 20140-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 20140-1-AP (MYH4-Specific Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 20140-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 20140-1-AP (MYH4-Specific Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse skeletal muscle using 20140-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20140-1-AP (MYH4-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse skeletal muscle using 20140-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using MYH4-Specific antibody (20140-1-AP) at dilution of 1:200, MYH2-specific antibody (55069-1-AP, green), MYH7-specific antibody (22280-1-AP, blue).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, humanes Skelettmuskelgewebe, Ratten-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Skelettmuskelgewebe, Maus-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Skelettmuskelgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 31 publications below
• IHC: See 2 publications below
• IF: See 10 publications below

Produktinformation

20140-1-AP bindet in WB, IHC, IF-P, ELISA MYH4-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Hund, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: myosin, heavy chain 4, skeletal muscle
• Berechnetes Molekulargewicht: 223 kDa
• Beobachtetes Molekulargewicht: 230 kDa
• GenBank-Zugangsnummer: NM_017533
• Gene symbol: MYH4
• Gene ID (NCBI): 4622
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Myosin is one of the most important skeletal muscle proteins. MYH4 functions muscle contraction. The antibody is specific to MYH4.

Publikationen

Species	Application	Title
rat	IHC,IF	Sci Adv; Bioactive fiber-reinforced hydrogel to tailor cell microenvironment for structural and functional regeneration of myotendinous junction; Authors - Yuzhi Sun
mouse	IF	Nat Commun; The nuclear envelope protein Net39 is essential for muscle nuclear integrity and chromatin organization.; Authors - Andres Ramirez-Martinez
mouse	IF	Development; The long noncoding RNA Meg3 regulates myoblast plasticity and muscle regeneration through epithelial-mesenchymal transition; Authors - Tiffany L. Dill
mouse	IF	Am J Pathol; Klotho null mutation involvement in adenosine A2B receptor-related skeletal muscle degeneration; Authors - Hung-Liang Pai
mouse	IF	Development; The long noncoding RNA Meg3 regulates myoblast plasticity and muscle regeneration through epithelial-mesenchymal transition.; Authors - Tiffany L Dill
mouse	WB	Int J Mol Sci; Loss of Myostatin Alters Mitochondrial Oxidative Phosphorylation, TCA Cycle Activity, and ATP Production in Skeletal Muscle; Authors - Xueqiao Wang