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MYH6 Polyklonaler Antikörper
MYH6 Polyklonal Antikörper für WB, IHC, IF-P, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte und mehr (4)
Anwendung
WB, IHC, IF-P, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 22281-1-AP
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | Mausherzgewebe, rat heart |
Erfolgreiche Detektion in IHC | humanes Herzgewebe, Mausherzgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF-P | Mausherzgewebe |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunhistochemie (IHC) | IHC : 1:20-1:200 |
Immunfluoreszenz (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 2 publications below |
WB | See 33 publications below |
IHC | See 5 publications below |
IF | See 17 publications below |
Produktinformation
22281-1-AP bindet in WB, IHC, IF-P, ELISA MYH6 und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Hausschwein, Huhn, Maus, Ratte, Rind, Ziege |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | Peptid |
Vollständiger Name | myosin, heavy chain 6, cardiac muscle, alpha |
Berechnetes Molekulargewicht | 224 kDa |
Beobachtetes Molekulargewicht | 200-220 kDa |
GenBank-Zugangsnummer | NM_002471 |
Gene symbol | MYH6 |
Gene ID (NCBI) | 4624 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
MYH6 encodes the alpha-cardiac myosin heavy chain (aMHC) which is a major contractile protein exclusively expressed in the myocardium of the heart. Expression of MYH6 is down-regulated in cardiac myopathy and failing hearts. Defects in MYH6 lead to congenital heart disease (CHD), and a spectrum of phenotypes ranging from hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM). (15737621, 20656787)
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for MYH6 antibody 22281-1-AP | Protokoll herunterladen |
IHC protocol for MYH6 antibody 22281-1-AP | Protokoll herunterladenl |
IF protocol for MYH6 antibody 22281-1-AP | Protokoll herunterladen |
IP protocol for MYH6 antibody 22281-1-AP | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Science HEART DISEASE. Titin mutations in iPS cells define sarcomere insufficiency as a cause of dilated cardiomyopathy. | ||
Dev Cell Force Generation via β-Cardiac Myosin, Titin, and α-Actinin Drives Cardiac Sarcomere Assembly from Cell-Matrix Adhesions. | ||
Elife Independent regulation of Z-lines and M-lines during sarcomere assembly in cardiac myocytes revealed by the automatic image analysis software sarcApp
| ||
Stem Cell Reports Distinctive Roles of Canonical and Noncanonical Wnt Signaling in Human Embryonic Cardiomyocyte Development. | ||
J Pathol Doxorubicin-induced cardiotoxicity involves IFNγ-mediated metabolic reprogramming in cardiomyocytes. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Charlène (Verified Customer) (04-16-2025) | The antibody is working well on our samples
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FH Hua (Verified Customer) (01-20-2023) | Very good antibody. Strong signal and clean background in WB.
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FH Jane (Verified Customer) (02-03-2022) | one specific band around 230kDa, however, the band was not very sharp. Probably will help if run at a lower percentage gel.
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FH Jing (Verified Customer) (02-08-2021) | good antibody, clear sigle band around 260KD.
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