MYH6 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MYH6 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

36

Rezensionen (3)

Kat-Nr. : 22281-1-AP

Synonyme

alpha MHC, ASD3, MYH6, MYHC, MyHC alpha, MYHCA, Myosin 6, Myosin heavy chain 6

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Galerie der Validierungsdaten

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  WB analysis using 22281-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22281-1-AP (MYH6 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 22281-1-AP

  IP Result of anti-MYH6 (IP:22281-1-AP, 5ug; Detection:22281-1-AP 1:300) with mouse heart tissue lysate 4000ug.

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  IHC staining of human heart using 22281-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22281-1-AP (MYH6 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 22281-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22281-1-AP (MYH6 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse heart using 22281-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22281-1-AP (MYH6 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse heart using 22281-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22281-1-AP (MYH6 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of mouse heart using 22281-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 22281-1-AP (MYH6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, rat heart
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Mausherzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 20 publications below
• IHC: See 4 publications below
• IF: See 12 publications below

Produktinformation

22281-1-AP bindet in WB, IHC, IF, ELISA MYH6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: myosin, heavy chain 6, cardiac muscle, alpha
• Berechnetes Molekulargewicht: 224 kDa
• Beobachtetes Molekulargewicht: 200-220 kDa
• GenBank-Zugangsnummer: NM_002471
• Gene symbol: MYH6
• Gene ID (NCBI): 4624
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYH6 encodes the alpha-cardiac myosin heavy chain (aMHC) which is a major contractile protein exclusively expressed in the myocardium of the heart. Expression of MYH6 is down-regulated in cardiac myopathy and failing hearts. Defects in MYH6 lead to congenital heart disease (CHD), and a spectrum of phenotypes ranging from hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM). (15737621, 20656787)

Publikationen

Species	Application	Title
human	WB	Science; HEART DISEASE. Titin mutations in iPS cells define sarcomere insufficiency as a cause of dilated cardiomyopathy.; Authors - John T Hinson
		Dev Cell; Force Generation via β-Cardiac Myosin, Titin, and α-Actinin Drives Cardiac Sarcomere Assembly from Cell-Matrix Adhesions.; Authors - Anant Chopra
human	WB	Elife; Independent regulation of Z-lines and M-lines during sarcomere assembly in cardiac myocytes revealed by the automatic image analysis software sarcApp; Authors - Abigail C Neininger-Castro ; KD Validated
human	IF	Stem Cell Reports; Distinctive Roles of Canonical and Noncanonical Wnt Signaling in Human Embryonic Cardiomyocyte Development.; Authors - Silvia Mazzotta
mouse		J Pathol; Doxorubicin-induced cardiotoxicity involves IFNγ-mediated metabolic reprogramming in cardiomyocytes.; Authors - Chen Ni
chicken	WB,IF	Int J Mol Sci; Circular RNA circIGF2BP3 Promotes the Proliferation and Differentiation of Chicken Primary Myoblasts; Authors - Xiaotong Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hua (Verified Customer) (01-20-2023)

Very good antibody. Strong signal and clean background in WB.

• Applications: Western Blot
• Primary Antibody Dilution: 1:8000
• Cell Tissue Type: Mouse heart

MYH6 Antibody Western Blot validation (1:8000 dilution) in Mouse heart (Cat no:22281-1-AP)

FH

Jane (Verified Customer) (02-03-2022)

one specific band around 230kDa, however, the band was not very sharp. Probably will help if run at a lower percentage gel.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Heart tissue

FH

Jing (Verified Customer) (02-08-2021)

good antibody, clear sigle band around 260KD.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse heart