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  • KD/KO Validated

MYH6 Polyklonaler Antikörper

MYH6 Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (4)

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(54)

Kat-Nr. : 22281-1-AP

Synonyme

Myosin 6, Myosin-6, Myosin heavy chain 6, MyHC-alpha, MYHCA



Geprüfte Anwendungen

Erfolgreiche Detektion in WBMausherzgewebe, rat heart
Erfolgreiche Detektion in IHChumanes Herzgewebe, Mausherzgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF-PMausherzgewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunhistochemie (IHC)IHC : 1:20-1:200
Immunfluoreszenz (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

22281-1-AP bindet in WB, IHC, IF-P, ELISA MYH6 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Hausschwein, Huhn, Maus, Ratte, Rind, Ziege
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name myosin, heavy chain 6, cardiac muscle, alpha
Berechnetes Molekulargewicht 224 kDa
Beobachtetes Molekulargewicht 200-220 kDa
GenBank-ZugangsnummerNM_002471
Gene symbol MYH6
Gene ID (NCBI) 4624
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYH6 encodes the alpha-cardiac myosin heavy chain (aMHC) which is a major contractile protein exclusively expressed in the myocardium of the heart. Expression of MYH6 is down-regulated in cardiac myopathy and failing hearts. Defects in MYH6 lead to congenital heart disease (CHD), and a spectrum of phenotypes ranging from hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM). (15737621, 20656787)

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for MYH6 antibody 22281-1-APProtokoll herunterladen
IHC protocol for MYH6 antibody 22281-1-APProtokoll herunterladenl
IF protocol for MYH6 antibody 22281-1-APProtokoll herunterladen
IP protocol for MYH6 antibody 22281-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Science

HEART DISEASE. Titin mutations in iPS cells define sarcomere insufficiency as a cause of dilated cardiomyopathy.

Authors - John T Hinson
mouse,ratWB

Cell Death Dis

Asb10 accelerates pathological cardiac remodeling by stabilizing HSP70

Authors - Ke Lin

Dev Cell

Force Generation via β-Cardiac Myosin, Titin, and α-Actinin Drives Cardiac Sarcomere Assembly from Cell-Matrix Adhesions.

Authors - Anant Chopra
humanWB

Elife

Independent regulation of Z-lines and M-lines during sarcomere assembly in cardiac myocytes revealed by the automatic image analysis software sarcApp

Authors - Abigail C Neininger-Castro
  • KD Validated
humanIF

Stem Cell Reports

Distinctive Roles of Canonical and Noncanonical Wnt Signaling in Human Embryonic Cardiomyocyte Development.

Authors - Silvia Mazzotta
mouse

J Pathol

Doxorubicin-induced cardiotoxicity involves IFNγ-mediated metabolic reprogramming in cardiomyocytes.

Authors - Chen Ni

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Charlène (Verified Customer) (04-16-2025)

The antibody is working well on our samples

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: cardiomyocytes derived from iPS
MYH6 Antibody Western Blot validation (1:1000 dilution) in cardiomyocytes derived from iPS (Cat no:22281-1-AP)
FH

Hua (Verified Customer) (01-20-2023)

Very good antibody. Strong signal and clean background in WB.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:8000
  • Cell Tissue Type: Mouse heart
MYH6 Antibody Western Blot validation (1:8000 dilution) in Mouse heart (Cat no:22281-1-AP)
FH

Jane (Verified Customer) (02-03-2022)

one specific band around 230kDa, however, the band was not very sharp. Probably will help if run at a lower percentage gel.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Heart tissue
FH

Jing (Verified Customer) (02-08-2021)

good antibody, clear sigle band around 260KD.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse heart