MYH6 Polyklonaler Antikörper

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• KD/KO Validated

MYH6 Polyklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 22281-1-PBS

Synonyme

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  WB analysis using 22281-1-AP (same clone as 22281-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22281-1-AP (MYH6 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22281-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse heart using 22281-1-AP (same clone as 22281-1-PBS)

  IP result of anti-MYH6 (IP:22281-1-AP, 5ug; Detection:22281-1-AP 1:300) with mouse heart tissue lysate 4000ug. This data was developed using the same antibody clone with 22281-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 22281-1-AP (same clone as 22281-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22281-1-AP (MYH6 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22281-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 22281-1-AP (same clone as 22281-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22281-1-AP (MYH6 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22281-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 22281-1-AP (same clone as 22281-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22281-1-AP (MYH6 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22281-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 22281-1-AP (same clone as 22281-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22281-1-AP (MYH6 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22281-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse heart using 22281-1-AP (same clone as 22281-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 22281-1-AP (MYH6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22281-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

22281-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA MYH6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: myosin, heavy chain 6, cardiac muscle, alpha
• Berechnetes Molekulargewicht: 224 kDa
• Beobachtetes Molekulargewicht: 200-220 kDa
• GenBank-Zugangsnummer: NM_002471
• Gene symbol: MYH6
• Gene ID (NCBI): 4624
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYH6 encodes the alpha-cardiac myosin heavy chain (aMHC) which is a major contractile protein exclusively expressed in the myocardium of the heart. Expression of MYH6 is down-regulated in cardiac myopathy and failing hearts. Defects in MYH6 lead to congenital heart disease (CHD), and a spectrum of phenotypes ranging from hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM). (15737621, 20656787)