MYH8-Specific Rekombinanter Antikörper

MYH8-Specific Rekombinant Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

241539A1

Kat-Nr. : 84594-1-RR

Synonyme

MYH8, 241539A1, Myosin-8, Myosin heavy chain 8, Myosin 8

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  WB analysis of rat skeletal muscle using 84594-1-RR

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 84594-1-RR (MYH8-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 84594-1-RR

  human heart tissue were subjected to SDS PAGE followed by western blot with 84594-1-RR (MYH8-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse skeletal muscle using 84594-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84594-1-RR (MYH8-Specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 84594-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84594-1-RR (MYH8-Specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse skeletal muscle using 84594-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using MYH8-Specific antibody (84594-1-RR, Clone: 241539A1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse skeletal muscle using 84594-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using MYH8-Specific antibody (84594-1-RR, Clone: 241539A1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Ratten-Skelettmuskelgewebe, humanes Herzgewebe
• Erfolgreiche Detektion in IHC: Maus-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Skelettmuskelgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)-P: IF-P : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84594-1-RR bindet in WB, IHC, IF-P, ELISA MYH8-Specific und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: myosin, heavy chain 8, skeletal muscle, perinatal
• Berechnetes Molekulargewicht: 223 kDa
• Beobachtetes Molekulargewicht: 220-230 kDa
• GenBank-Zugangsnummer: NM_002472
• Gene symbol: MYH8
• Gene ID (NCBI): 4626
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.