MYH9 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MYH9 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

29

Kat-Nr. : 11128-1-AP

Synonyme

MYH9, Myosin 9, Myosin heavy chain 9, myosin IIa, Nonmuscle myosin IIa

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Galerie der Validierungsdaten

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  WB analysis using 11128-1-AP

  Various cell lysates were subjected to SDS PAGE followed by western blot with 11128-1-AP (MYH9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11128-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11128-1-AP (MYH9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 11128-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11128-1-AP (MYH9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 11128-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11128-1-AP (MYH9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 11128-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 11128-1-AP (MYH9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 11128-1-AP

  IP Result of anti-MYH9 (IP:11128-1-AP, 5ug; Detection:11128-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human lung cancer using 11128-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11128-1-AP (MYH9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11128-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11128-1-AP (MYH9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 11128-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11128-1-AP (MYH9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 11128-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11128-1-AP (MYH9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 11128-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11128-1-AP (MYH9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of mouse small intestine using 11128-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using MYH9 antibody (11128-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 11128-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MYH9 antibody (11128-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 11128-1-AP

  Immunofluorescent analysis of HepG2 cells, using MYH9 antibody 11128-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  FC experiment of HepG2 using 11128-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human MYH9 (11128-1-AP) and Fluorescein (FITC)-conjugated Affinipure Goat Anti-Rabbit IgG(H+L) at dilution 1:200 (red), or 0.2 ug Control Antibody. Cells were fixed with 90% MeOH .

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, humanes Hirngewebe, Maushirngewebe, Maus-Kolongewebe, Mausnierengewebe, U2OS-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Nierengewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maus-Dünndarmgewebe, HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 22 publications below
• IHC: See 7 publications below
• IF: See 16 publications below
• IP: See 3 publications below
• CoIP: See 3 publications below

Produktinformation

11128-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ELISA MYH9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MYH9 fusion protein Ag1612
• Vollständiger Name: myosin, heavy chain 9, non-muscle
• Berechnetes Molekulargewicht: 227 kDa
• Beobachtetes Molekulargewicht: 224 kDa
• GenBank-Zugangsnummer: BC011915
• Gene symbol: MYH9
• Gene ID (NCBI): 4627
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYH9 (Myosin-9) appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. MYH9 is a hexameric protein that consists of two heavy chains (~200 kDa) and two pairs of non-identical light chains (15-22 kDa), a pair of essential or alkali light chains, and a pair of regulatory light chains (PMID: 12800156).

Publikationen

Species	Application	Title
human	WB,IF	Acta Pharm Sin B; Ginsenoside Rg1 ameliorates blood-brain barrier disruption and traumatic brain injury via attenuating macrophages derived exosomes miR-21 release.; Authors - Kefeng Zhai
human	WB	J Exp Clin Cancer Res; ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK; Authors - Li Cui
human	WB,IF	Cell Death Differ; Glycoprotein PTGDS promotes tumorigenesis of diffuse large B-cell lymphoma by MYH9-mediated regulation of Wnt-β-catenin-STAT3 signaling; Authors - Shunfeng Hu ; KD Validated
human,mouse	WB,IHC,IF	Cell Rep; Mechanotransduction in response to ECM stiffening impairs cGAS immune signaling in tumor cells; Authors - Yingqi Liu
mouse	IF,CoIP,WB	Theranostics; Salvia miltiorrhiza-derived miRNAs suppress vascular remodeling through regulating OTUD7B/KLF4/NMHC IIA axis.; Authors - Gao-Shan Yang
mouse	IF,WB	Stem Cell Reports; Non-muscle myosin heavy chain 9 maintains intestinal homeostasis by preventing epithelium necroptosis and colitis adenoma formation.; Authors - Shan Wang ; KO Validated

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