MYH9 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

MYH9 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(11)

CloneNo.

5D9D2

Kat-Nr. : 60233-1-Ig

Synonyme

5D9D2, Myosin 9, Myosin heavy chain 9, Myosin heavy chain, non-muscle IIa, Myosin-9

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  WB analysis of mouse kidney using 60233-1-Ig

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 60233-1-Ig (MYH9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 60233-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60233-1-Ig (MYH9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 60233-1-Ig

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 60233-1-Ig (MYH9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig pancreas using 60233-1-Ig

  pig pancreas tissue were subjected to SDS PAGE followed by western blot with 60233-1-Ig (MYH9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 60233-1-Ig

  human kidney tissue were subjected to SDS PAGE followed by western blot with 60233-1-Ig (MYH9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig spleen using 60233-1-Ig

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 60233-1-Ig (MYH9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 60233-1-Ig

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 60233-1-Ig (MYH9 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 60233-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 60233-1-Ig (MYH9 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of U2OS using 60233-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using 60233-1-Ig (MYH9 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, A431-Zellen, humanes Nierengewebe, humanes Plazenta-Gewebe, Maus-Kolongewebe, Hausschwein-Nierengewebe, Hausschwein-Pankreasgewebe, Hausschwein-Milzgewebe, U2OS-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 7 publications below
• CoIP: See 1 publications below

Produktinformation

60233-1-Ig bindet in WB, IHC, IF/ICC, CoIP, ELISA MYH9 und zeigt Reaktivität mit Hausschwein, human, Maus

• Getestete Reaktivität: Hausschwein, human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MYH9 fusion protein Ag1612
• Vollständiger Name: myosin, heavy chain 9, non-muscle
• Berechnetes Molekulargewicht: 227 kDa
• Beobachtetes Molekulargewicht: 230 kDa, 160 kDa
• GenBank-Zugangsnummer: BC011915
• Gene symbol: MYH9
• Gene ID (NCBI): 4627
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYH9 (Myosin-9) appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. MYH9 is a hexameric protein that consists of two heavy chains (~200 kDa) and two pairs of non-identical light chains (15-22 kDa), a pair of essential or alkali light chains, and a pair of regulatory light chains (PMID: 12800156).

Publikationen

Species	Application	Title
human	WB	Cell Stem Cell; Mechano-oncogenic cytoskeletal remodeling drives leukemic transformation with mitochondrial vesicle-mediated STING activation; Authors - Zemin Song
human	WB	Nat Commun; Lymphocyte-specific protein 1 regulates mechanosensory oscillation of podosomes and actin isoform-based actomyosin symmetry breaking.; Authors - Pasquale Cervero
human	WB,IHC,IF	EBioMedicine; PMID: 31594754; Authors - Yiyi Liu ; KD Validated
human	WB,IF,CoIP	Cell Death Differ; Glycoprotein PTGDS promotes tumorigenesis of diffuse large B-cell lymphoma by MYH9-mediated regulation of Wnt-β-catenin-STAT3 signaling; Authors - Shunfeng Hu ; KD Validated
mouse,human	WB,IHC,IF	Cell Rep; Mechanotransduction in response to ECM stiffening impairs cGAS immune signaling in tumor cells; Authors - Yingqi Liu
mouse	IF	Theranostics; Salvia miltiorrhiza-derived miRNAs suppress vascular remodeling through regulating OTUD7B/KLF4/NMHC IIA axis.; Authors - Gao-Shan Yang