MYL3 Monoklonaler Antikörper

MYL3 Monoklonal Antikörper für IF/ICC, IF-P

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

IF/ICC, IF-P

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2E4B2

Kat-Nr. : CL488-66286

Synonyme

Myosin light chain 3, Myosin light chain 1, slow-twitch muscle B/ventricular isoform, MYL 3, MLC-lV/sb, MLC1V

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  IF Staining of mouse skeletal muscle using CL488-66286

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using CL488-66286 (MYL3 antibody) at dilution of 1:100.

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  IF Staining of mouse skeletal muscle using CL488-66286

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using CL488-66286 (MYL3 antibody) at dilution of 1:100.

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  IF Staining of C2C12 using CL488-66286

  Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using CL488-66286 (MYL3 antibody) at dilution of 1:100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IF/ICC: C2C12-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66286 bindet in IF/ICC, IF-P MYL3 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MYL3 fusion protein Ag24535
• Vollständiger Name: myosin, light chain 3, alkali; ventricular, skeletal, slow
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 22-25 kDa
• GenBank-Zugangsnummer: BC009790
• Gene symbol: MYL3
• Gene ID (NCBI): 4634
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYL3, also named as MLC1v, is an essential light chain of myosin that is associated with muscle contraction. It is expressed in ventricular and slow skeletal muscle. MYL3 may serve as a target for caspase-3 in dying cardiomyocytes. Mutations of MYL3 gene cause hypertrophic cardiomyopathy. MYL3 has been identified as potential serum biomarker for drug induced myotoxicity. Great increase in MYL3 serum concentration has been observed in rats with cardiac and skeletal muscle injury. (PMID:21685905)