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MYL6 Polyklonaler Antikörper

MYL6 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18158-1-PBS

Synonyme

MYL 6, MLC-3

Filter:
  • Western Blot (WB) analysis of various lysates using MYL6 Polyclonal antibody (18158-1-AP)

    WB analysis using 18158-1-AP (same clone as 18158-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18158-1-AP (MYL6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18158-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using MYL6 Polyclonal antibody (18158-1-AP)

    IHC staining of mouse heart using 18158-1-AP (same clone as 18158-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18158-1-AP (MYL6 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18158-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MYL6 Polyclonal antibody (18158-1-AP)

    IF Staining of HeLa using 18158-1-AP (same clone as 18158-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MYL6 antibody (18158-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18158-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

18158-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA MYL6 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen MYL6 fusion protein Ag12964
Vollständiger Name myosin, light chain 6, alkali, smooth muscle and non-muscle
Berechnetes Molekulargewicht 17 kDa
Beobachtetes Molekulargewicht15-20 kDa
GenBank-ZugangsnummerBC006781
Gene symbol MYL6
Gene ID (NCBI) 4637
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. MYL6 (Myosin light polypeptide 6), encodes a myosin alkali light chain that is expressed in smooth muscle and non-muscle tissues. Expression of MYL6 is high in fibroblasts and myoblasts (PMID:8188229, PubMed:2304459, PMID:2722814).