MYL6 Monoklonaler Antikörper

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• KD/KO Validated

MYL6 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2G4C9

Kat-Nr. : 68142-1-PBS

Synonyme

17 kDa myosin light chain, ESMLC, LC17, LC17 GI, LC17 NM, LC17A, LC17B, MLC 3, MLC1SM, MLC3NM, MLC3SM, MYL6, Myosin light chain 3, Myosin light chain A3, Myosin light chain alkali 3, Myosin light polypeptide 6

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  WB analysis using 68142-1-Ig (same clone as 68142-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68142-1-Ig (MYL6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68142-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 68142-1-Ig (same clone as 68142-1-PBS)

  WB result of MYL6 antibody (68142-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYL6 transfected A549 cells. This data was developed using the same antibody clone with 68142-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 68142-1-Ig (same clone as 68142-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 68142-1-Ig (MYL6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68142-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 68142-1-Ig (same clone as 68142-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 68142-1-Ig (MYL6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68142-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 68142-1-Ig (same clone as 68142-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68142-1-Ig (MYL6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68142-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 68142-1-Ig (same clone as 68142-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68142-1-Ig (MYL6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68142-1-PBS in a different storage buffer formulation.

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  IHC staining of rat heart using 68142-1-Ig (same clone as 68142-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 68142-1-Ig (MYL6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68142-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 68142-1-Ig (same clone as 68142-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68142-1-Ig (MYL6 antibody) at dilution of 1:2000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68142-1-PBS in a different storage buffer formulation.

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  IHC staining of rat skeletal muscle using 68142-1-Ig (same clone as 68142-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 68142-1-Ig (MYL6 antibody) at dilution of 1:2000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68142-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68142-1-Ig (same clone as 68142-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MYL6 antibody (68142-1-Ig, Clone: 2G4C9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68142-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68142-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA MYL6 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MYL6 fusion protein Ag13091
• Vollständiger Name: myosin, light chain 6, alkali, smooth muscle and non-muscle
• Berechnetes Molekulargewicht: 17 kDa
• Beobachtetes Molekulargewicht: 17-25 kDa
• GenBank-Zugangsnummer: BC006781
• Gene symbol: MYL6
• Gene ID (NCBI): 4637
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. MYL6 (Myosin light polypeptide 6), encodes a myosin alkali light chain that is expressed in smooth muscle and non-muscle tissues. Expression of MYL6 is high in fibroblasts and myoblasts (PMID:8188229, PubMed:2304459, PMID:2722814).