MYLK2 Polyklonaler Antikörper

MYLK2 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21173-1-PBS

Synonyme

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  WB analysis using 21173-1-AP (same clone as 21173-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21173-1-AP (MYLK2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21173-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 21173-1-AP (same clone as 21173-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 21173-1-AP (MYLK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21173-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human skeletal muscle using 21173-1-AP (same clone as 21173-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21173-1-AP (MYLK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21173-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 21173-1-AP (same clone as 21173-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21173-1-AP (MYLK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21173-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 21173-1-AP (same clone as 21173-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21173-1-AP (MYLK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21173-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 21173-1-AP (same clone as 21173-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21173-1-AP (MYLK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21173-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

21173-1-PBS bindet in WB, IHC, Indirect ELISA MYLK2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MYLK2 fusion protein Ag15424
• Vollständiger Name: myosin light chain kinase 2
• Berechnetes Molekulargewicht: 596 aa, 65 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC069627
• Gene symbol: MYLK2
• Gene ID (NCBI): 85366
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYLK2, also named as MLCK2, belongs to the protein kinase superfamily and CAMK Ser/Thr protein kinase family. MYLK2 catalyze the reaction: ATP + [myosin light-chain] = ADP + [myosin light-chain] phosphate. MYLK2 is implicated in the level of global muscle contraction and cardiac function. MYLK2 phosphorylates a specific serine in the N-terminus of a myosin light chain. Mutation of MYLK2 will cause the cardiomyopathy familial hypertrophic (CMH).