MYLK3 Polyklonaler Antikörper

MYLK3 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 21527-1-AP

Synonyme

Myosin light chain kinase 3, MLCK, EC:2.7.11.18, Cardiac-MyBP-C-associated Ca/CaM kinase, Cardiac-MLCK

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  WB analysis using 21527-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21527-1-AP (MYLK3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 21527-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 21527-1-AP (MYLK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 21527-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 21527-1-AP (MYLK3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon using 21527-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 21527-1-AP (MYLK3 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human heart using 21527-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21527-1-AP (MYLK3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 21527-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21527-1-AP (MYLK3 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 21527-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MYLK3 antibody (21527-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 21527-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 21527-1-AP (MYLK3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, humanes Herzgewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

21527-1-AP bindet in WB, IHC, IF/ICC, ELISA MYLK3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MYLK3 fusion protein Ag16137
• Vollständiger Name: myosin light chain kinase 3
• Berechnetes Molekulargewicht: 819 aa, 88 kDa
• Beobachtetes Molekulargewicht: 85-100 kDa
• GenBank-Zugangsnummer: BC109097
• Gene symbol: MYLK3
• Gene ID (NCBI): 91807
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cardiac myosin light chain kinase (cMLCK) encoded by the myosin light chain kinase 3 (MYLK3) phosphorylates cardiac myosin light chain 2 (MLC2, encoded by MYL2) and facilitates actin-myosin interactions that enhance contractile force in the animal heart (PMID: 29235529).

Publikationen

Species	Application	Title
rat	WB	Basic Res Cardiol; Alkaline nucleoplasm facilitates contractile gene expression in the mammalian heart.; Authors - Alzbeta Hulikova
human	WB	Front Pharmacol; GYY4137, as a slow-releasing H2S donor, ameliorates sodium deoxycholate-induced chronic intestinal barrier injury and gut microbiota dysbiosis; Authors - Shaorong Pan
mouse	WB	J Agric Food Chem; Bisdemethoxycurcumin and Curcumin Alleviate Inflammatory Bowel Disease by Maintaining Intestinal Epithelial Integrity and Regulating Gut Microbiota in Mice; Authors - Kai-Yu Hsu
human,mouse	IHC,WB,IF	Cell Commun Signal; GPR65 inhibits human trophoblast cell adhesion through upregulation of MYLK and downregulation of fibronectin via cAMP-ERK signaling in a low pH environment; Authors - Jia Mao
mouse	WB	Nat Commun; Capture of RNA-binding proteins across mouse tissues using HARD-AP; Authors - Yijia Ren