MYLK4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MYLK4 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 24309-1-AP

Synonyme

MLCK4, MYLK4, SgK085, Sugen kinase 85

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  WB analysis using 24309-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24309-1-AP (MYLK4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 24309-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24309-1-AP (MYLK4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 24309-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 24309-1-AP (MYLK4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 24309-1-AP

  IP result of anti-MYLK4 (IP:24309-1-AP, 4ug; Detection:24309-1-AP 1:500) with HEK-293 cells lysate 3600ug.

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  IHC staining of human heart using 24309-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24309-1-AP (MYLK4 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 24309-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24309-1-AP (MYLK4 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human tonsillitis using 24309-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24309-1-AP (MYLK4 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 24309-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24309-1-AP (MYLK4 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, L02-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

24309-1-AP bindet in WB, IHC, IF, IP, ELISA MYLK4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MYLK4 fusion protein Ag19314
• Vollständiger Name: myosin light chain kinase family, member 4
• Berechnetes Molekulargewicht: 388 aa, 45 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC132831
• Gene symbol: MYLK4
• Gene ID (NCBI): 340156
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYLK4(Myosin light chain kinase family member 4) is also named as SGK085 and belongs to the CAMK Ser/Thr protein kinase family. MLCKs are activated by Ca2+-calmodulin complexes and require Mg2+ or Ca2+ as a co-factor. It has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
	WB,IHC	Proc Natl Acad Sci U S A; Cardiac myosin light chain is phosphorylated by Ca2+/calmodulin-dependent and -independent kinase activities.; Authors - Audrey N Chang
human	WB	J Exp Clin Cancer Res; MYLK4 promotes tumor progression through the activation of epidermal growth factor receptor signaling in osteosarcoma.; Authors - Mengkai Yang ; KD Validated
	IF	bioRxiv; GATA1-defective immune-megakaryocytes as possible drivers of idiopathic pulmonary fibrosis; Authors - Francesca Gobbo