- Featured Product
- KD/KO Validated
MYO18A Polyklonaler Antikörper
MYO18A Polyklonal Antikörper für WB, IHC, IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte
Anwendung
WB, IHC, IF, FC (Intra), IP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 14611-1-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | Jurkat-Zellen, HeLa-Zellen, K-562-Zellen, Neuro-2a-Zellen, NIH/3T3-Zellen |
| Erfolgreiche IP | HeLa-Zellen |
| Erfolgreiche Detektion in IHC | Mausherzgewebe, Maus-Skelettmuskelgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunhistochemie (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 4 publications below |
| WB | See 14 publications below |
| IHC | See 1 publications below |
| IF | See 5 publications below |
| FC | See 1 publications below |
Produktinformation
14611-1-AP bindet in WB, IHC, IF, FC (Intra), IP, ELISA MYO18A und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte |
| In Publikationen genannte Reaktivität | human, Maus, Ratte |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | MYO18A fusion protein Ag6185 |
| Vollständiger Name | myosin XVIIIA |
| Berechnetes Molekulargewicht | 233 kDa |
| Beobachtetes Molekulargewicht | 230 kDa, 190 kDa |
| GenBank-Zugangsnummer | BC039612 |
| Gene symbol | MYO18A |
| Gene ID (NCBI) | 399687 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
MYO18A is an unconventional myosin that may play a role in the stabilization and organization of the actin cytoskeleton. Recently MYO18A has been identified as the receptor of SP and is responsible for clearance of SP-A-opsonized bacteria in in vivo infection. Two different isoforms of mouse Myo18A have been identified: Myo18A-alpha and Myo18A-beta as 230- and 190-kDa proteins, respectively. Myo18a-alpha colocalizes with the ER-Golgi complex and with membrane ruffles and filopodia, while Myo18a-beta localizes diffusely to the cytoplasm.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for MYO18A antibody 14611-1-AP | Protokoll herunterladen |
| IHC protocol for MYO18A antibody 14611-1-AP | Protokoll herunterladenl |
| IP protocol for MYO18A antibody 14611-1-AP | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
J Clin Invest A pro-tumorigenic secretory pathway activated by p53 deficiency in lung adenocarcinoma.
| ||
Cell Death Differ The Smad4-MYO18A-PP1A complex regulates β-catenin phosphorylation and pemigatinib resistance by inhibiting PAK1 in cholangiocarcinoma.
| ||
Thorax Advanced MicroRNA delivery for lung inflammatory therapy: surfactant protein A controls cellular internalisation and degradation of extracellular vesicles | ||
Cell Syst A Portrait of the Human Organelle Proteome In Space and Time during Cytomegalovirus Infection.
| ||
Oncoimmunology Identification of CD245 as myosin 18A, a receptor for surfactant A: A novel pathway for activating human NK lymphocytes. | ||
Diabetes Unbiased Profiling of the Human Proinsulin Biosynthetic Interaction Network Reveals a Role For Peroxiredoxin 4 in Proinsulin Folding. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Sammy (Verified Customer) (04-22-2024) | Detects several bands. One at the correct MW that will need to be validated by KO/KD.
|









