MYO18A Polyklonaler Antikörper

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• KD/KO Validated

MYO18A Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14611-1-PBS

Synonyme

CD245, KIAA0216, Molecule associated with JAK3 N-terminus, myosin XVIIIA, myosinXVIIIA

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  WB analysis of Jurkat using 14611-1-AP (same clone as 14611-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14611-1-AP (MYO18A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14611-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 14611-1-AP (same clone as 14611-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14611-1-AP (MYO18A Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14611-1-PBS in a different storage buffer formulation.

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  WB analysis of Neuro-2a using 14611-1-AP (same clone as 14611-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 14611-1-AP (MYO18A Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14611-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 14611-1-AP (same clone as 14611-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 14611-1-AP (MYO18A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14611-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 14611-1-AP (same clone as 14611-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14611-1-AP (MYO18A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14611-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 14611-1-AP (same clone as 14611-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14611-1-AP (MYO18A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14611-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 14611-1-AP (same clone as 14611-1-PBS)

  IP result of anti-MYO18A (IP:14611-1-AP, 3ug; Detection:14611-1-AP 1:300) with HeLa cells lysate 3000ug. This data was developed using the same antibody clone with 14611-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 14611-1-AP (same clone as 14611-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14611-1-AP (MYO18A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14611-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 14611-1-AP (same clone as 14611-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14611-1-AP (MYO18A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14611-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 14611-1-AP (same clone as 14611-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14611-1-AP (MYO18A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14611-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14611-1-PBS bindet in WB, IHC, IP, Indirect ELISA MYO18A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MYO18A fusion protein Ag6185
• Vollständiger Name: myosin XVIIIA
• Berechnetes Molekulargewicht: 233 kDa
• Beobachtetes Molekulargewicht: 230 kDa, 190 kDa
• GenBank-Zugangsnummer: BC039612
• Gene symbol: MYO18A
• Gene ID (NCBI): 399687
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYO18A is an unconventional myosin that may play a role in the stabilization and organization of the actin cytoskeleton. Recently MYO18A has been identified as the receptor of SP and is responsible for clearance of SP-A-opsonized bacteria in in vivo infection. Two different isoforms of mouse Myo18A have been identified: Myo18A-alpha and Myo18A-beta as 230- and 190-kDa proteins, respectively. Myo18a-alpha colocalizes with the ER-Golgi complex and with membrane ruffles and filopodia, while Myo18a-beta localizes diffusely to the cytoplasm.