MYO19 Polyklonaler Antikörper

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• KD/KO Validated

MYO19 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 23906-1-AP

Synonyme

MYO19, MYOHD1, myosin XIX

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  WB analysis of HeLa using 23906-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23906-1-AP (MYO19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 23906-1-AP

  WB result of MYO19 antibody (23906-1-AP, 1:300) with si-Control and si-MYO19 transfected A549 cells.

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  WB analysis of A549 using 23906-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 23906-1-AP (MYO19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COS-7 using 23906-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 23906-1-AP (MYO19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 23906-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23906-1-AP (MYO19 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 23906-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 23906-1-AP (MYO19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 23906-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 23906-1-AP (MYO19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 23906-1-AP

  Immunofluorescent analysis of A549 cells using 23906-1-AP (MYO19 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, COS-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, Rattennierengewebe, Rattenhodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

23906-1-AP bindet in WB, IHC, IF/ICC, ELISA MYO19 und zeigt Reaktivität mit Affe, human, Ratten

• Getestete Reaktivität: Affe, human, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MYO19 fusion protein Ag20995
• Vollständiger Name: myosin XIX
• Berechnetes Molekulargewicht: 970 aa, 109 kDa
• Beobachtetes Molekulargewicht: 109 kDa
• GenBank-Zugangsnummer: BC008900
• Gene symbol: MYO19
• Gene ID (NCBI): 80179
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYO19 is also named as MYOHD1. It is Actin-based motor molecule with ATPase activity that localizes to the mitochondrion outer membrane (PubMed:19932026, PubMed:23568824, PubMed:25447992) and required for mitochondrial inheritance during mitosis (PubMed:25447992). MYO19 can enable actin binding activity and be involved in regulation of cytokinesis and regulation of mitochondrial fission. MYO19 can be detected as about 109 kDa.

Publikationen

Species	Application	Title
	WB	Sci Adv; ARMC1 partitions between distinct complexes and assembles MIRO with MTFR to control mitochondrial distribution; Authors - Michael J McKenna
human,mouse	WB,IHC,IF	J Pharm Anal; Coral calcium hydride promotes peripheral mitochondrial division and reduces AT-II cells damage in ARDS via activation of the Trx2/Myo19/Drp1 pathway; Authors - Qian Li