MYO1A Polyklonaler Antikörper

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• KD/KO Validated

MYO1A Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 17499-1-AP

Synonyme

MIHC, DFNA48, Brush border myosin I, BBM-I, BBMI

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  WB analysis of HepG2 using 17499-1-AP

  WB result of MYO1A antibody (17499-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYO1A transfected HepG2 cells.

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  WB analysis of mouse brain using 17499-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17499-1-AP (MYO1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17499-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17499-1-AP (MYO1A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17499-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17499-1-AP (MYO1A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 17499-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17499-1-AP (MYO1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17499-1-AP

  IP result of anti-MYO1A (IP:17499-1-AP, 4ug; Detection:17499-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IHC staining of human colon cancer using 17499-1-AP

  Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 17499-1-AP (MYO1A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 17499-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17499-1-AP (MYO1A antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 17499-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17499-1-AP (MYO1A antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 17499-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MYO1A antibody (17499-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of HeLa using 17499-1-AP

  Immunofluorescent analysis of Hela cells, using MYO1A antibody 17499-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, A431-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

17499-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA MYO1A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MYO1A fusion protein Ag11682
• Vollständiger Name: myosin IA
• Berechnetes Molekulargewicht: 1043 aa, 118 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC059387
• Gene symbol: MYO1A
• Gene ID (NCBI): 4640
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
	WB	Cell Rep Med; Personalized drug screening using patient-derived organoid and its clinical relevance in gastric cancer; Authors - Yi Zhao
mouse	WB	Clin Sci (Lond); A new mechanism of arterial calcification in diabetes: interaction between H3K18 lactylation and CHI3L1; Authors - Yi Zhu