MYO1E Polyklonaler Antikörper

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• KD/KO Validated

MYO1E Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 17768-1-AP

Synonyme

HuncM IC, MYO1E, Myosin Ic, myosin IE

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  WB analysis using 17768-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17768-1-AP (MYO1E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17768-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17768-1-AP (MYO1E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17768-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17768-1-AP (MYO1E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 17768-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17768-1-AP (MYO1E antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-1080.Zellen, HeLa-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

17768-1-AP bindet in WB, IHC, IF, ELISA MYO1E und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MYO1E fusion protein Ag12176
• Vollständiger Name: myosin IE
• Berechnetes Molekulargewicht: 1108 aa, 127 kDa
• Beobachtetes Molekulargewicht: 127 kDa
• GenBank-Zugangsnummer: BC098392
• Gene symbol: MYO1E
• Gene ID (NCBI): 4643
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYO1E, also named as Myosin-Ie and MYO1C (distinct from the MYO1C gene located on chromosome 17), encodes a member of the nonmuscle class I myosins which are a subgroup of the unconventional myosin protein family. The unconventional myosin proteins function as actin-based molecular motors. This protein localizes to the cytoplasm and may be involved in intracellular movement and membrane trafficking. Mutations in this gene are the cause of focal segmental glomerulosclerosis-6.

Publikationen

Species	Application	Title
mouse	WB	Front Immunol; Myo1f has an essential role in γδT intraepithelial lymphocyte adhesion and migration; Authors - Irving Ulises Martínez-Vargas
mouse	WB	Eur J Immunol; The class I myosin Myo1e regulates TLR4-triggered macrophage spreading, chemokine release and antigen presentation via MHC class II.; Authors - Jens Wenzel ; KO Validated
		iScience; Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.; Authors - Mio Iwasaki
human	WB,IF	Oncol Lett; Analysis and validation of the potential of the MYO1E gene in pancreatic adenocarcinoma based on a bioinformatics approach; Authors - Songbai Liu ; KD Validated
mouse	WB	Exp Neurol; Roles of Adam8 in Neuroinflammation in experimental ischemic Stroke: Insights from single-cell and ribosome-bound mRNA sequencing; Authors - Jiale Liu ; KD Validated