MYO1G Polyklonaler Antikörper

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• KD/KO Validated

MYO1G Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25772-1-AP

Synonyme

HA 2, Minor histocompatibility antigen HA-2, myosin IG, Unconventional myosin-Ig

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  WB analysis of Jurkat using 25772-1-AP

  WB result of MYO1G antibody (25772-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYO1G transfected Jurkat cells.

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  WB analysis using 25772-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25772-1-AP (MYO1G antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of Jurkat using 25772-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using MYO1G antibody (25772-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IF/ICC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

25772-1-AP bindet in WB, IF/ICC, ELISA MYO1G und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MYO1G fusion protein Ag22696
• Vollständiger Name: myosin IG
• Berechnetes Molekulargewicht: 1018 aa, 116 kDa
• Beobachtetes Molekulargewicht: 110-120 kDa
• GenBank-Zugangsnummer: BC113544
• Gene symbol: MYO1G
• Gene ID (NCBI): 64005
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYO1G is a plasma membrane-associated class I myosin that is abundant in T and B lymphocytes and mast cells and expressed specifically in the haematopoietic system. Class I myosins have been implicated in various cellular processes relying on actin-dependent membrane dynamics such as endocytosis, secretion, adhesion, motility and regulation of mechanosensitive channels (PMID: 19968988). MYO1G localizes to the plasma membrane linked to PIP2 and PIP3, is particularly enriched at cell-surface microvilli, and associates in an ATP-releasable manner to the actin cytoskeleton (PMID: 24310084). MYO1G can be detected as about 110-120 kDa.