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MYO1G Polyklonaler Antikörper

MYO1G Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25772-1-PBS

Synonyme

HA 2, Minor histocompatibility antigen HA-2, myosin IG, Unconventional myosin-Ig

Filter:
  • Western Blot (WB) analysis of Jurkat cells using MYO1G Polyclonal antibody (25772-1-AP)

    WB analysis of Jurkat using 25772-1-AP (same clone as 25772-1-PBS)

    WB result of MYO1G antibody (25772-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYO1G transfected Jurkat cells. This data was developed using the same antibody clone with 25772-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MYO1G Polyclonal antibody (25772-1-AP)

    WB analysis using 25772-1-AP (same clone as 25772-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25772-1-AP (MYO1G antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25772-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using MYO1G Polyclonal antibody (25772-1-AP)

    IF Staining of Jurkat using 25772-1-AP (same clone as 25772-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using MYO1G antibody (25772-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25772-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

25772-1-PBS bindet in WB, IF/ICC, Indirect ELISA MYO1G und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen MYO1G fusion protein Ag22696
Vollständiger Name myosin IG
Berechnetes Molekulargewicht 1018 aa, 116 kDa
Beobachtetes Molekulargewicht110-120 kDa
GenBank-ZugangsnummerBC113544
Gene symbol MYO1G
Gene ID (NCBI) 64005
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYO1G is a plasma membrane-associated class I myosin that is abundant in T and B lymphocytes and mast cells and expressed specifically in the haematopoietic system. Class I myosins have been implicated in various cellular processes relying on actin-dependent membrane dynamics such as endocytosis, secretion, adhesion, motility and regulation of mechanosensitive channels (PMID: 19968988). MYO1G localizes to the plasma membrane linked to PIP2 and PIP3, is particularly enriched at cell-surface microvilli, and associates in an ATP-releasable manner to the actin cytoskeleton (PMID: 24310084). MYO1G can be detected as about 110-120 kDa.