MYO1G Rekombinanter Antikörper

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• KD/KO Validated

MYO1G Rekombinant Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240503E1

Kat-Nr. : 83556-5-RR

Synonyme

240503E1, HA 2, Minor histocompatibility antigen HA-2, myosin IG, Unconventional myosin-Ig

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  WB analysis using 83556-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83556-5-RR (MYO1G antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 83556-5-RR

  WB result of MYO1G antibody (83556-5-RR; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYO1G transfected Jurkat cells.

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  IF Staining of Jurkat using 83556-5-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using MYO1G antibody (83556-5-RR, Clone: 240503E1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of Jurkat using 83556-5-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using MYO1G antibody (83556-5-RR, Clone: 240503E1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of Jurkat using 83556-5-RR

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug MYO1G Recombinant antibody (83556-5-RR, Clone:240503E1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83556-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83556-5-RR against Human MYO1G were performed. The affinity constant is 1.03 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, Ramos-Zellen
• Erfolgreiche Detektion in IF/ICC: Jurkat-Zellen
• Erfolgreiche Detektion in FC (Intra): Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83556-5-RR bindet in WB, IF/ICC, FC (Intra), ELISA MYO1G und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MYO1G fusion protein Ag22698
• Vollständiger Name: myosin IG
• Berechnetes Molekulargewicht: 1018 aa, 116 kDa
• Beobachtetes Molekulargewicht: 110-120 kDa
• GenBank-Zugangsnummer: BC113544
• Gene symbol: MYO1G
• Gene ID (NCBI): 64005
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYO1G is a plasma membrane-associated class I myosin that is abundant in T and B lymphocytes and mast cells and expressed specifically in the haematopoietic system. Class I myosins have been implicated in various cellular processes relying on actin-dependent membrane dynamics such as endocytosis, secretion, adhesion, motility and regulation of mechanosensitive channels (PMID: 19968988). MYO1G localizes to the plasma membrane linked to PIP2 and PIP3, is particularly enriched at cell-surface microvilli, and associates in an ATP-releasable manner to the actin cytoskeleton (PMID: 24310084). MYO1G can be detected as about 110-120 kDa.