MYO1G Rekombinanter Antikörper

MYO1G Rekombinant Antikörper für IF/ICC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240503E1

Kat-Nr. : CL488-83556-5

Synonyme

240503E1, HA 2, Minor histocompatibility antigen HA-2, myosin IG, Unconventional myosin-Ig

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  IF Staining of Jurkat using CL488-83556-5

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite® Plus 488 MYO1G antibody (CL488-83556-5, Clone: 240503E1 ) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: Jurkat-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-83556-5 bindet in IF/ICC MYO1G und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MYO1G fusion protein Ag22698
• Vollständiger Name: myosin IG
• Berechnetes Molekulargewicht: 1018 aa, 116 kDa
• Beobachtetes Molekulargewicht: 110-120 kDa
• GenBank-Zugangsnummer: BC113544
• Gene symbol: MYO1G
• Gene ID (NCBI): 64005
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYO1G is a plasma membrane-associated class I myosin that is abundant in T and B lymphocytes and mast cells and expressed specifically in the haematopoietic system. Class I myosins have been implicated in various cellular processes relying on actin-dependent membrane dynamics such as endocytosis, secretion, adhesion, motility and regulation of mechanosensitive channels (PMID: 19968988). MYO1G localizes to the plasma membrane linked to PIP2 and PIP3, is particularly enriched at cell-surface microvilli, and associates in an ATP-releasable manner to the actin cytoskeleton (PMID: 24310084). MYO1G can be detected as about 110-120 kDa.