MYO5B Polyklonaler Antikörper

MYO5B Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29996-1-PBS

Synonyme

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  WB analysis using 29996-1-AP (same clone as 29996-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29996-1-AP (MYO5B antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29996-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 29996-1-AP (same clone as 29996-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29996-1-AP (MYO5B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29996-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 29996-1-AP (same clone as 29996-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29996-1-AP (MYO5B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29996-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 29996-1-AP (same clone as 29996-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 29996-1-AP (MYO5B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29996-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

29996-1-PBS bindet in WB, IHC, Indirect ELISA MYO5B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MYO5B fusion protein Ag31590
• Vollständiger Name: myosin VB
• Berechnetes Molekulargewicht: 214KD
• Beobachtetes Molekulargewicht: 200 kDa
• GenBank-Zugangsnummer: NM_001080467
• Gene symbol: MYO5B
• Gene ID (NCBI): 4645
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYO5B is a member of the class V of unconventional, dimeric nonfilamentous myosins, which may be involved in vesicular trafficking. Members of the Rab family of small GTPases, consisting of Rab11a, Rab11b, and Rab25, have recently emerged as potential mediators of vesicle transport by MYO5B (PMID: 21282656). MYO5B is required for polarization of hepatocytes and mutations of MYO5B could disrupt epithelial cell polarity. Loss-of-Function of MYO5B caused by mutation is the main cause of microvillus inclusion disease (MVID). Recently it was reported that the expression of MYO5B was downregulated in gastric cancer and inactivation of MYO5B may contribute to tumorigenesis (PMID: 22134786).