MYO7A Polyklonaler Antikörper

MYO7A Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 20720-1-AP

Synonyme

DFNA11, DFNB2, MYO7A, myosin VIIA, MYOVIIA, MYU7A, NSRD2, USH1B

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Galerie der Validierungsdaten

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  WB analysis of L02 using 20720-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20720-1-AP (MYO7A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 20720-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 20720-1-AP (MYO7A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 20720-1-AP

  Immunofluorescent analysis of HepG2 cells, using MYO7A antibody 20720-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, A431-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 3 publications below

Produktinformation

20720-1-AP bindet in WB, IF, ELISA MYO7A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: myosin VIIA
• Berechnetes Molekulargewicht: 254 kDa
• Beobachtetes Molekulargewicht: 160-255 kDa
• GenBank-Zugangsnummer: NM_000260
• Gene symbol: MYO7A
• Gene ID (NCBI): 4647
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYO7A, also named a USH1B, is one of myosins protein which are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. In retina, MYO7A might play a role in trafficking of ribbon-synaptic vesicle complexes and renewal of the outer photoreceptors disks. In inner ear, it might maintain the rigidity of stereocilia during the dynamic movements of the bundle. It is involved in hair-cell vesicle trafficking of aminoglycosides, which are known to induce ototoxicity. Defects in MYO7A are the cause of Usher syndrome type 1B (USH1B). Defects in MYO7A are the cause of deafness autosomal recessive type 2 (DFNB2). Defects in MYO7A are the cause of deafness autosomal dominant type 11 (DFNA11). The antibody is specific to MYO7A.

Publikationen

Species	Application	Title
human	IF	ACS Biomater Sci Eng; Microenvironment Can Induce Development of Auditory Progenitor Cells from Human Gingival Mesenchymal Stem Cells.; Authors - Sevda Pouraghaei
zebrafish	IF	Biomedicines; Knockout of mafba Causes Inner-Ear Developmental Defects in Zebrafish via the Impairment of Proliferation and Differentiation of Ionocyte Progenitor Cells.; Authors - Xiang Chen
human	WB,IF	Mol Biol Cell; Comparative analysis of the MyTH4-FERM myosins reveals insights into the determinants of actin track selection in polarized epithelia.; Authors - Samaneh Matoo