MYO7A Polyklonaler Antikörper

MYO7A Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20720-1-PBS

Synonyme

DFNA11, DFNB2, myosin VIIA, MYOVIIA, MYU7A

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  WB analysis of L02 using 20720-1-AP (same clone as 20720-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 20720-1-AP (MYO7A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20720-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 20720-1-AP (same clone as 20720-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 20720-1-AP (MYO7A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20720-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 20720-1-AP (same clone as 20720-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20720-1-AP (MYO7A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20720-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 20720-1-AP (same clone as 20720-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20720-1-AP (MYO7A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20720-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 20720-1-AP (same clone as 20720-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20720-1-AP (MYO7A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20720-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 20720-1-AP (same clone as 20720-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20720-1-AP (MYO7A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20720-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 20720-1-AP (same clone as 20720-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MYO7A antibody (20720-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 20720-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

20720-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA MYO7A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: myosin VIIA
• Berechnetes Molekulargewicht: 254 kDa
• Beobachtetes Molekulargewicht: 240-250 kDa
• GenBank-Zugangsnummer: NM_000260
• Gene symbol: MYO7A
• Gene ID (NCBI): 4647
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYO7A, also named a USH1B, is one of myosins protein which are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. In retina, MYO7A might play a role in trafficking of ribbon-synaptic vesicle complexes and renewal of the outer photoreceptors disks. In inner ear, it might maintain the rigidity of stereocilia during the dynamic movements of the bundle. It is involved in hair-cell vesicle trafficking of aminoglycosides, which are known to induce ototoxicity. Defects in MYO7A are the cause of Usher syndrome type 1B (USH1B). Defects in MYO7A are the cause of deafness autosomal recessive type 2 (DFNB2). Defects in MYO7A are the cause of deafness autosomal dominant type 11 (DFNA11). The antibody is specific to MYO7A.