MYO7A Rekombinanter Antikörper

MYO7A Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240612C11

Kat-Nr. : 83807-1-PBS

Synonyme

240612C11, DFNA11, DFNB2, myosin VIIA, MYOVIIA

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  WB analysis using 83807-1-RR (same clone as 83807-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83807-1-RR (MYO7A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83807-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 83807-1-RR (same clone as 83807-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83807-1-RR (MYO7A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83807-1-PBS in a different storage buffer formulation.

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  IF Staining of Y79 using 83807-1-RR (same clone as 83807-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Y79 cells using MYO7A antibody (83807-1-RR, Clone: 240612C11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83807-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 83807-1-RR (same clone as 83807-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug MYO7A Recombinant antibody (83807-1-RR, Clone:240612C11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83807-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

83807-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), ELISA MYO7A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: myosin VIIA
• Berechnetes Molekulargewicht: 254 kDa
• Beobachtetes Molekulargewicht: 254 kDa
• GenBank-Zugangsnummer: NM_000260
• Gene symbol: MYO7A
• Gene ID (NCBI): 4647
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYO7A, also named a USH1B, is one of myosins protein which are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. In retina, MYO7A might play a role in trafficking of ribbon-synaptic vesicle complexes and renewal of the outer photoreceptors disks. In inner ear, it might maintain the rigidity of stereocilia during the dynamic movements of the bundle. It is involved in hair-cell vesicle trafficking of aminoglycosides, which are known to induce ototoxicity. Defects in MYO7A are the cause of Usher syndrome type 1B (USH1B). Defects in MYO7A are the cause of deafness autosomal recessive type 2 (DFNB2). Defects in MYO7A are the cause of deafness autosomal dominant type 11 (DFNA11). The antibody is specific to MYO7A.