Myocilin Polyklonaler Antikörper

Myocilin Polyklonal Antikörper für IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 14238-1-AP

Synonyme

GLC1A, GPOA, JOAG, JOAG1, MYOC, Myocilin, TIGR

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Galerie der Validierungsdaten

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  WB analysis of human heart using 14238-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14238-1-AP (Myocilin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 14238-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14238-1-AP (Myocilin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human stomach using 14238-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 14238-1-AP (Myocilin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 14238-1-AP

  IP Result of anti-Myocilin (IP:14238-1-AP, 4ug; Detection:14238-1-AP 1:500) with mouse skeletal muscle tissue lysate 4000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Herzgewebe, humanes Magengewebe, Maus-Skelettmuskelgewebe
• Erfolgreiche IP: Maus-Skelettmuskelgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

14238-1-AP bindet in WB, IP, ELISA Myocilin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Myocilin fusion protein Ag5492
• Vollständiger Name: myocilin, trabecular meshwork inducible glucocorticoid response
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 66 kDa
• GenBank-Zugangsnummer: BC029261
• Gene symbol: MYOC
• Gene ID (NCBI): 4653
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Myocilin (MYOC), also known as TIGR or GLC1A, is a secreted glycoprotein of the olfactomedin family, originally identified in trabecular meshwork cells after prolonged treatment with glucocorticoids, and, independently, in the retina (PMID: 9176893; 9169133). It may participate in the obstruction of fluid outflow in the trabecular meshwork. Defects in MYOC are the cause of primary open angle glaucoma (POAG) (PMID: 21709622). MYOC has a molecular weight of 55-57 kDa, besides, a 66-kDa form, which could be a result of post-translational modifications, has also been reported in some researches (PMID: 9497363; 12150989).

Publikationen

Species	Application	Title
human	WB	Ann Transl Med; Adenosine A3 receptor activated in H2O2 oxidative stress of primary open-angle glaucoma.; Authors - Ziyu Zhou
human	WB	Int J Ophthalmol; Expression profile analysis to identify potential gene changes induced by dexamethasone in the trabecular meshwork; Authors - Miao Wei
mouse	WB	J Ocul Pharmacol Ther; Activation of Sonic Hedgehog Signaling Pathway Regulates Human Trabecular Meshwork Cell Function; Authors - Xiaochen Wang