Myocilin Monoklonaler Antikörper

Myocilin Monoklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

CloneNo.

4B1F6

Kat-Nr. : 60357-1-Ig

Synonyme

GLC1A, GPOA, JOAG, JOAG1, MYOC, myocilin, TIGR

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Galerie der Validierungsdaten

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  WB analysis of pig heart using 60357-1-Ig

  pig heart tissue were subjected to SDS PAGE followed by western blot with 60357-1-Ig (Myocilin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 60357-1-Ig

  rat heart tissue were subjected to SDS PAGE followed by western blot with 60357-1-Ig (Myocilin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 60357-1-Ig

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 60357-1-Ig (Myocilin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 60357-1-Ig

  human spleen tissue were subjected to SDS PAGE followed by western blot with 60357-1-Ig (Myocilin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 60357-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60357-1-Ig (Myocilin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 60357-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60357-1-Ig (Myocilin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 60357-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 60357-1-Ig (Myocilin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 60357-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 60357-1-Ig (Myocilin antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Herzgewebe, humanes Milzgewebe, humanes Skelettmuskelgewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

60357-1-Ig bindet in WB, IHC, IF, ELISA Myocilin und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Myocilin fusion protein Ag5492
• Vollständiger Name: myocilin, trabecular meshwork inducible glucocorticoid response
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 50-57 kDa
• GenBank-Zugangsnummer: BC029261
• Gene symbol: MYOC
• Gene ID (NCBI): 4653
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Myocilin (MYOC), also known as TIGR or GLC1A, is a secreted glycoprotein of the olfactomedin family, originally identified in trabecular meshwork cells after prolonged treatment with glucocorticoids, and, independently, in the retina (PMID: 9176893; 9169133). It may participate in the obstruction of fluid outflow in the trabecular meshwork. Defects in MYOC are the cause of primary open angle glaucoma (POAG) (PMID: 21709622).

Publikationen

Species	Application	Title
	IHC	Stem Cell Res Ther; Human amniotic epithelial cell transplantation improves scar remodeling in a rabbit model of acute vocal fold injury: a pilot study.; Authors - Yourka D Tchoukalova
rat	WB	Exp Eye Res; Sympathetic activation leads to Schlemm's canal expansion via increasing vasoactive intestinal polypeptide secretion from trabecular meshwork; Authors - Dingwen Xu
human	WB,IF	Int Ophthalmol; The effect of ET1-CTGF mediated pathway on the accumulation of extracellular matrix in the trabecular meshwork and its contribution to the increase in IOP; Authors - Junming Wang
human	WB	Transl Vis Sci Technol; Inhibition of TGF-β2-Induced Trabecular Meshwork Fibrosis by Pirfenidone; Authors - Xiaofeng Zhu
mouse	IF	Res Sq; Lentiviral mediated delivery of CRISPR/Cas9 reduces intraocular pressure in a mouse model of myocilin glaucoma; Authors - Shruti V Patil
human	WB	Biochem Biophys Res Commun; Identification and application of p75 neurotrophin receptor-expressing human trabecular meshwork progenitor cells.; Authors - Susumu Hara