MYOD1 Polyklonaler Antikörper

MYOD1 Polyklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (7)

Anwendung

WB, IHC, IF, FC, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

83

Rezensionen (1)

Kat-Nr. : 18943-1-AP

Synonyme

bHLHc1, Myf 3, MYF3, MYOD, MYOD1, myogenic differentiation 1, Myogenic factor 3, PUM

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Galerie der Validierungsdaten

• 

  WB analysis using 18943-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18943-1-AP (MYOD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18943-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18943-1-AP (MYOD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 18943-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 18943-1-AP (MYOD1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 18943-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18943-1-AP (MYOD1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 18943-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18943-1-AP (MYOD1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 18943-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 18943-1-AP (MYOD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse embryo using 18943-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 18943-1-AP (MYOD1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse embryo using 18943-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 18943-1-AP (MYOD1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse embryo using 18943-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 18943-1-AP (MYOD1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of C2C12 using 18943-1-AP

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human MYOD1 (18943-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, humanes Herzgewebe, Maus-Skelettmuskelgewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: Mausembryogewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: C2C12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 64 publications below
• IHC: See 10 publications below
• IF: See 21 publications below
• ChIP: See 1 publications below

Produktinformation

18943-1-AP bindet in WB, IHC, IF, FC, ChIP, ELISA MYOD1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ente, Hausschwein, Huhn, Kaninchen, Maus, Ratte, Rind, Zebrafisch, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MYOD1 fusion protein Ag13512
• Vollständiger Name: myogenic differentiation 1
• Berechnetes Molekulargewicht: 320 aa, 35 kDa
• Beobachtetes Molekulargewicht: 35-45 kDa
• GenBank-Zugangsnummer: BC064493
• Gene symbol: MYOD1
• Gene ID (NCBI): 4654
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYOD1, also named as BHLHC1 or MYF3, is a 320 amino acid protein, which promotes the transcriptional activity of MYOD1 through its CDK9-mediated phosphorylation. This phosphorylation promotes its function in muscle differentiation. MYOD1 acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. MYOD1 together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. he calcualted molecular weight of MYOD1 is 34 kDa, but modified MYOD1 is about 45 kDa. (PMID: 12037670)

Publikationen

Species	Application	Title
human	IF	Nature; A plant-derived natural photosynthetic system for improving cell anabolism; Authors - Pengfei Chen
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
	WB	Nature; SAR1B senses leucine levels to regulate mTORC1 signalling.; Authors - Jie Chen
mouse	WB	Nat Commun; The transcription factor NF-Y participates to stem cell fate decision and regeneration in adult skeletal muscle.; Authors - Giovanna Rigillo
mouse	WB	J Cachexia Sarcopenia Muscle; circRNAome profiling reveals circFgfr2 regulates myogenesis and muscle regeneration via a feedback loop.; Authors - Junyu Yan
human	WB,IHC	Cancer Res; YAP1-mediated suppression of USP31 enhances NF-κB activity to promote sarcomagenesis.; Authors - Shuai Ye

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Cassandra (Verified Customer) (02-04-2021)

This antibody worked quite well in my IF staining. I used 1 to 100 antibody dilution.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Satellite cell

MYOD1 Antibody Immunofluorescence validation (1:100 dilution) in Satellite cell (Cat no:18943-1-AP)

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