MYOD1 Polyklonaler Antikörper

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• KD/KO Validated

MYOD1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18943-1-PBS

Synonyme

bHLHc1, Class C basic helix-loop-helix protein 1, Myf 3, MYF3, Myf-3

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  WB analysis using 18943-1-AP (same clone as 18943-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18943-1-AP (MYOD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18943-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 18943-1-AP (same clone as 18943-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18943-1-AP (MYOD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18943-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 18943-1-AP (same clone as 18943-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 18943-1-AP (MYOD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18943-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat heart using 18943-1-AP (same clone as 18943-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 18943-1-AP (MYOD1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18943-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skeletal muscle using 18943-1-AP (same clone as 18943-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18943-1-AP (MYOD1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18943-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 18943-1-AP (same clone as 18943-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18943-1-AP (MYOD1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18943-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse embryo using 18943-1-AP (same clone as 18943-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 18943-1-AP (MYOD1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18943-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse embryo using 18943-1-AP (same clone as 18943-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 18943-1-AP (MYOD1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18943-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse embryo using 18943-1-AP (same clone as 18943-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 18943-1-AP (MYOD1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18943-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 18943-1-AP (same clone as 18943-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MYOD1 antibody (18943-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 18943-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

18943-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA MYOD1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MYOD1 fusion protein Ag13512
• Vollständiger Name: myogenic differentiation 1
• Berechnetes Molekulargewicht: 320 aa, 35 kDa
• Beobachtetes Molekulargewicht: 35-45 kDa
• GenBank-Zugangsnummer: BC064493
• Gene symbol: MYOD1
• Gene ID (NCBI): 4654
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYOD1, also named as BHLHC1 or MYF3, is a 320 amino acid protein, which promotes the transcriptional activity of MYOD1 through its CDK9-mediated phosphorylation. This phosphorylation promotes its function in muscle differentiation. MYOD1 acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. MYOD1 together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. he calcualted molecular weight of MYOD1 is 34 kDa, but modified MYOD1 is about 45 kDa. (PMID: 12037670)