MYOF Polyklonaler Antikörper

MYOF Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19548-1-PBS

Synonyme

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  WB analysis using 19548-1-AP (same clone as 19548-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19548-1-AP (MYOF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19548-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 19548-1-AP (same clone as 19548-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 19548-1-AP (Myoferlin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19548-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 19548-1-AP (same clone as 19548-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 19548-1-AP (Myoferlin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19548-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 19548-1-AP (same clone as 19548-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19548-1-AP (Myoferlin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19548-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 19548-1-AP (same clone as 19548-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19548-1-AP (Myoferlin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19548-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 19548-1-AP (same clone as 19548-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Myoferlin antibody (19548-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 19548-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

19548-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA MYOF und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: myoferlin
• Berechnetes Molekulargewicht: 235 kDa
• Beobachtetes Molekulargewicht: 235-250 kDa
• GenBank-Zugangsnummer: NM_133337
• Gene symbol: MYOF
• Gene ID (NCBI): 26509
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYOF, also named as FER1L3 and KIAA1207, belongs to the ferlin family. MYOF is a calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. It is involved in endocytic recycling. MYOF is implicated in VEGF signal transduction by regulating the levels of the receptor KDR. The antibody can recognize all the 8 isoforms of MYOF.