MYOM3 Polyklonaler Antikörper

MYOM3 Polyklonal Antikörper für WB, IHC, IF-P, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17692-1-PBS

Synonyme

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  WB analysis using 17692-1-AP (same clone as 17692-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17692-1-AP (MYOM3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17692-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse skeletal muscle using 17692-1-AP (same clone as 17692-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17692-1-AP (MYOM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17692-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skeletal muscle using 17692-1-AP (same clone as 17692-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17692-1-AP (MYOM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17692-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse skeletal muscle using 17692-1-AP (same clone as 17692-1-PBS)

  IP result of anti-MYOM3 (IP:17692-1-AP, 4ug; Detection:17692-1-AP 1:1000) with mouse skeletal muscle tissue lysate 1280 ug. This data was developed using the same antibody clone with 17692-1-PBS in a different storage buffer formulation.

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  IHC staining of human skeletal muscle using 17692-1-AP (same clone as 17692-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17692-1-AP (MYOM3 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 17692-1-PBS in a different storage buffer formulation.

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  IHC staining of human skeletal muscle using 17692-1-AP (same clone as 17692-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17692-1-AP (MYOM3 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 17692-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 17692-1-AP (same clone as 17692-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 17692-1-AP (MYOM3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17692-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 17692-1-AP (same clone as 17692-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 17692-1-AP (MYOM3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17692-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse skeletal muscle using 17692-1-AP (same clone as 17692-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using MYOM3 antibody (17692-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17692-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

17692-1-PBS bindet in WB, IHC, IF-P, IP, Indirect ELISA MYOM3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MYOM3 fusion protein Ag11849
• Vollständiger Name: myomesin family, member 3
• Berechnetes Molekulargewicht: 162 kDa, 136 kDa
• Beobachtetes Molekulargewicht: 162 kDa
• GenBank-Zugangsnummer: BC067101
• Gene symbol: MYOM3
• Gene ID (NCBI): 127294
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYOM3 (myomesin 3) is a structural component of the M-band in striated muscle and is involved in sarcomere stability and resistance during intense or sustained stretching. MYOM3 can be detected mainly in intermediate-speed fibers of skeletal muscle. Recently high levels of MYOM3 fragments were detected in sera from patients with muscular dystrophy, including Duchenne muscular dystrophy (DMD). MYOM3 fragments may be used as serum biomarkers for DMD and other neuromuscular disorders. This antibody recognizes the intact MYOM3 protein (160-162 kDa) as well as MYOM3 fragments (100 kDa and 130 kDa). (26060189)