MYOT Polyklonaler Antikörper

MYOT Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 10731-1-AP

Synonyme

57 kDa cytoskeletal protein, LGMD1, LGMD1A, MYOT, myotilin, TTID

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Galerie der Validierungsdaten

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  WB analysis of mouse skeletal muscle using 10731-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10731-1-AP (MYOT antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 10731-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10731-1-AP (MYOT antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 10731-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10731-1-AP (MYOT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse skeletal muscle using 10731-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 10731-1-AP (MYOT antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 10731-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 10731-1-AP (MYOT antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, Mausherzgewebe
• Erfolgreiche Detektion in IHC: Maus-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

10731-1-AP bindet in WB, IHC, IF, ELISA MYOT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MYOT fusion protein Ag1112
• Vollständiger Name: myotilin
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 55-57 kDa, 35 kDa
• GenBank-Zugangsnummer: BC005376
• Gene symbol: MYOT
• Gene ID (NCBI): 9499
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYOT (myotilin) is a structural protein of the striated muscle Z-discs. It interacts with both actinin and filamin, forming a complex to maintain structural stability of muscles. In adult tissues, myotilin is mainly expressed in skeletal and cardiac muscles and in the peripheral nerves. Missense mutations of myotilin cause limb girdle muscular dystrophy 1A and some other myopathy. Two isoforms of MYOT exist due to the alternative splicing. This antibody can detect both of isoforms around 57 kDa and 35 kDa.

Publikationen

Species	Application	Title
human	IHC	Neurology; Mutations in HSPB8 causing a new phenotype of distal myopathy and motor neuropathy.; Authors - Roula Ghaoui
human	WB	Am J Pathol; In vivo characterization of mutant myotilins.; Authors - Keduka Etsuko E
		Neurol Genet; Novel mutation in TNPO3 causes congenital limb-girdle myopathy with slow progression.; Authors - Anna Vihola
mouse	IHC	Elife; Isoform-specific mutation in Dystonin-b gene causes late-onset protein aggregate myopathy and cardiomyopathy; Authors - Nozomu Yoshioka
human	IF	Eur J Neurol; Novel mutations in DNAJB6 cause LGMD1D and distal myopathy in French families; Authors - P H Jonson
human	IF	Ann Neurol; Actininopathy: A new muscular dystrophy caused by ACTN2 dominant mutations.; Authors - Marco Savarese