MYOT Polyklonaler Antikörper

MYOT Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10731-1-PBS

Synonyme

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  WB analysis of mouse skeletal muscle using 10731-1-AP (same clone as 10731-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10731-1-AP (MYOT antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10731-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skeletal muscle using 10731-1-AP (same clone as 10731-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10731-1-AP (MYOT antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10731-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 10731-1-AP (same clone as 10731-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10731-1-AP (MYOT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10731-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 10731-1-AP (same clone as 10731-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 10731-1-AP (MYOT antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10731-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 10731-1-AP (same clone as 10731-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 10731-1-AP (MYOT antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10731-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10731-1-PBS bindet in WB, IHC, Indirect ELISA MYOT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MYOT fusion protein Ag1112
• Vollständiger Name: myotilin
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 55-57 kDa, 35 kDa
• GenBank-Zugangsnummer: BC005376
• Gene symbol: MYOT
• Gene ID (NCBI): 9499
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYOT (myotilin) is a structural protein of the striated muscle Z-discs. It interacts with both actinin and filamin, forming a complex to maintain structural stability of muscles. In adult tissues, myotilin is mainly expressed in skeletal and cardiac muscles and in the peripheral nerves. Missense mutations of myotilin cause limb girdle muscular dystrophy 1A and some other myopathy. Two isoforms of MYOT exist due to the alternative splicing. This antibody can detect both of isoforms around 57 kDa and 35 kDa.